Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro
and inside cells. In addition to aligning individual particles, accurate registration of sample …

AreTomo, an abbreviation for Alignment and Reconstruction for Electron Tomography, is a
GPU accelerated software package that fully automates motion-corrected marker-free …

Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of
macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner …

Structural biology studies inside cells and tissues require methods to thin vitrified specimens
to electron transparency. Until now, focused ion beams based on gallium have been used …

Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …