Production and properties of microbial inulinases: recent advances
N Kango, SC Jain - Food Biotechnology, 2011 - Taylor & Francis
Microbial inulinases find application in enzymatic hydrolysis of inulin for production of
fructose (sweetener) and inulooligosaccharides (prebiotics). Inulin is one of the most …
fructose (sweetener) and inulooligosaccharides (prebiotics). Inulin is one of the most …
Extremophiles: from abyssal to terrestrial ecosystems and possibly beyond
F Canganella, J Wiegel - Naturwissenschaften, 2011 - Springer
The anthropocentric term “extremophile” was introduced more than 30 years ago to describe
any organism capable of living and growing under extreme conditions—ie, particularly …
any organism capable of living and growing under extreme conditions—ie, particularly …
Strategies for protein synthetic biology
R Grünberg, L Serrano - Nucleic acids research, 2010 - academic.oup.com
Proteins are the most versatile among the various biological building blocks and a mature
field of protein engineering has lead to many industrial and biomedical applications. But the …
field of protein engineering has lead to many industrial and biomedical applications. But the …
Thermophilic proteins as versatile scaffolds for protein engineering
AJ Finch, JR Kim - Microorganisms, 2018 - mdpi.com
Literature from the past two decades has outlined the existence of a trade-off between
protein stability and function. This trade-off creates a unique challenge for protein engineers …
protein stability and function. This trade-off creates a unique challenge for protein engineers …
Protein engineering strategies to expand CRISPR‐Cas9 applications
LF Ribeiro, LFC Ribeiro, MQ Barreto… - International journal of …, 2018 - Wiley Online Library
The development of precise and modulated methods for customized manipulation of DNA is
an important objective for the study and engineering of biological processes and is essential …
an important objective for the study and engineering of biological processes and is essential …
A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination
Background Saccharification of lignocellulosic material by xylanases and other glycoside
hydrolases is generally conducted at high concentrations of the final reaction products …
hydrolases is generally conducted at high concentrations of the final reaction products …
A designed bifunctional laccase/β-1, 3–1, 4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse
A bifunctional enzyme has been created by fusing two Bacillus subtilis enzymes: the β-1, 3–
1, 4-glucanase (BglS, EC 3.2. 1.73) that hydrolyzes plant cell wall β-glucans and the copper …
1, 4-glucanase (BglS, EC 3.2. 1.73) that hydrolyzes plant cell wall β-glucans and the copper …
Converting a periplasmic binding protein into a synthetic biosensing switch through domain insertion
LF Ribeiro, V Amarelle, LFC Ribeiro… - BioMed Research …, 2019 - Wiley Online Library
All biosensing platforms rest on two pillars: specific biochemical recognition of a particular
analyte and transduction of that recognition into a readily detectable signal. Most existing …
analyte and transduction of that recognition into a readily detectable signal. Most existing …
Laboratory Evolution of Bacillus circulans Xylanase Inserted into Pyrococcus furiosus Maltodextrin-Binding Protein for Increased Xylanase Activity and Thermal …
High xylanase activity and stability toward alkaline pH is strongly desired for pulping and
bleaching processes. We previously enhanced thermal stability of Bacillus circulans …
bleaching processes. We previously enhanced thermal stability of Bacillus circulans …
Stability of a guest protein depends on stability of a host protein in insertional fusion
B Pierre, T Xiong, L Hayles… - Biotechnology and …, 2011 - Wiley Online Library
Insertional fusion between host and guest protein domains has been employed to create
multi‐domain protein complexes displaying integrated and coupled functionalities. The …
multi‐domain protein complexes displaying integrated and coupled functionalities. The …