Production and properties of microbial inulinases: recent advances

N Kango, SC Jain - Food Biotechnology, 2011 - Taylor & Francis
Microbial inulinases find application in enzymatic hydrolysis of inulin for production of
fructose (sweetener) and inulooligosaccharides (prebiotics). Inulin is one of the most …

Extremophiles: from abyssal to terrestrial ecosystems and possibly beyond

F Canganella, J Wiegel - Naturwissenschaften, 2011 - Springer
The anthropocentric term “extremophile” was introduced more than 30 years ago to describe
any organism capable of living and growing under extreme conditions—ie, particularly …

Strategies for protein synthetic biology

R Grünberg, L Serrano - Nucleic acids research, 2010 - academic.oup.com
Proteins are the most versatile among the various biological building blocks and a mature
field of protein engineering has lead to many industrial and biomedical applications. But the …

Thermophilic proteins as versatile scaffolds for protein engineering

AJ Finch, JR Kim - Microorganisms, 2018 - mdpi.com
Literature from the past two decades has outlined the existence of a trade-off between
protein stability and function. This trade-off creates a unique challenge for protein engineers …

Protein engineering strategies to expand CRISPR‐Cas9 applications

LF Ribeiro, LFC Ribeiro, MQ Barreto… - International journal of …, 2018 - Wiley Online Library
The development of precise and modulated methods for customized manipulation of DNA is
an important objective for the study and engineering of biological processes and is essential …

A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination

LF Ribeiro, J Tullman, N Nicholes, SRB Silva… - Biotechnology for …, 2016 - Springer
Background Saccharification of lignocellulosic material by xylanases and other glycoside
hydrolases is generally conducted at high concentrations of the final reaction products …

A designed bifunctional laccase/β-1, 3–1, 4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

GP Furtado, LF Ribeiro, MR Lourenzoni… - … Engineering, Design & …, 2013 - academic.oup.com
A bifunctional enzyme has been created by fusing two Bacillus subtilis enzymes: the β-1, 3–
1, 4-glucanase (BglS, EC 3.2. 1.73) that hydrolyzes plant cell wall β-glucans and the copper …

Converting a periplasmic binding protein into a synthetic biosensing switch through domain insertion

LF Ribeiro, V Amarelle, LFC Ribeiro… - BioMed Research …, 2019 - Wiley Online Library
All biosensing platforms rest on two pillars: specific biochemical recognition of a particular
analyte and transduction of that recognition into a readily detectable signal. Most existing …

Laboratory Evolution of Bacillus circulans Xylanase Inserted into Pyrococcus furiosus Maltodextrin-Binding Protein for Increased Xylanase Activity and Thermal …

V Shah, T Charlton, JR Kim - Applied biochemistry and biotechnology, 2018 - Springer
High xylanase activity and stability toward alkaline pH is strongly desired for pulping and
bleaching processes. We previously enhanced thermal stability of Bacillus circulans …

Stability of a guest protein depends on stability of a host protein in insertional fusion

B Pierre, T Xiong, L Hayles… - Biotechnology and …, 2011 - Wiley Online Library
Insertional fusion between host and guest protein domains has been employed to create
multi‐domain protein complexes displaying integrated and coupled functionalities. The …