Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects

D Ballard, J Winkler-Galicki, J Wesoły - International Journal of Legal …, 2020 - Springer
In the last decade, next-generation sequencing (NGS) technology, alternatively massive
parallel sequencing (MPS), was applied to all fields of biological research. Its introduction to …

[HTML][HTML] Mitochondrial DNA heteroplasmy in the emerging field of massively parallel sequencing

RS Just, JA Irwin, W Parson - Forensic Science International: Genetics, 2015 - Elsevier
Long an important and useful tool in forensic genetic investigations, mitochondrial DNA
(mtDNA) typing continues to mature. Research in the last few years has demonstrated both …

[HTML][HTML] Developmental validation of the MiSeq FGx forensic genomics system for targeted next generation sequencing in forensic DNA casework and database …

AC Jäger, ML Alvarez, CP Davis, E Guzmán… - Forensic Science …, 2017 - Elsevier
Human DNA profiling using PCR at polymorphic short tandem repeat (STR) loci followed by
capillary electrophoresis (CE) size separation and length-based allele typing has been the …

Maternal age effect and severe germ-line bottleneck in the inheritance of human mitochondrial DNA

B Rebolledo-Jaramillo, MSW Su… - Proceedings of the …, 2014 - National Acad Sciences
The manifestation of mitochondrial DNA (mtDNA) diseases depends on the frequency of
heteroplasmy (the presence of several alleles in an individual), yet its transmission across …

Antibiotic-Induced, Increased Conjugative Transfer Is Common to Diverse Naturally Occurring ESBL Plasmids in Escherichia coli

G Liu, K Bogaj, V Bortolaia, JE Olsen… - Frontiers in …, 2019 - frontiersin.org
Previously, we showed that cefotaxime (CTX) exposure increases conjugative transfer of a
bla CTX–M–1 encoding IncI1 plasmid (IncI1/pST49/CTX-M-1) in Escherichia coli in a SOS …

Diversity of the gut microbiota in dihydrotestosterone-induced PCOS rats and the pharmacologic effects of diane-35, probiotics, and berberine

F Zhang, T Ma, P Cui, A Tamadon, S He… - Frontiers in …, 2019 - frontiersin.org
Polycystic ovary syndrome (PCOS) is a frequent endocrine and metabolic syndrome in
reproductive-age women. Recently, emerging evidence has shown that gut microbiota is …

Evaluation of the Illumina ForenSeq™ DNA Signature Prep Kit–MPS forensic application for the MiSeq FGx™ benchtop sequencer

C Xavier, W Parson - Forensic Science International: Genetics, 2017 - Elsevier
Abstract Massively Parallel (Next Generation) Sequencing (MPS) technologies have
recently been proven useful and successful in typing various markers relevant in forensic …

Validation of NGS for mitochondrial DNA casework at the FBI Laboratory

MD Brandhagen, RS Just, JA Irwin - Forensic Science International …, 2020 - Elsevier
As a first step towards integrating next generation sequencing (NGS) technology into the FBI
Laboratory's operational casework, the PowerSeq™ CRM Nested System, an NGS-based …

Evaluation of the precision ID whole MtDNA genome panel for forensic analyses

C Strobl, M Eduardoff, MM Bus, M Allen… - Forensic Science …, 2018 - Elsevier
Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using
an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher …

Massively parallel sequencing of complete mitochondrial genomes from hair shaft samples

W Parson, G Huber, L Moreno, MB Madel… - Forensic Science …, 2015 - Elsevier
Though shed hairs are one of the most commonly encountered evidence types, they are
among the most limited in terms of DNA quantity and quality. As a result, DNA testing has …