The use of force probes to induce unfolding and refolding of single molecules through the
application of mechanical tension, known as single-molecule force spectroscopy (SMFS) …

Single-molecule force spectroscopy with the atomic force microscope provides molecular
level insights into protein function, allowing researchers to reconstruct energy landscapes …

SARS-CoV-2 has been spreading around the world for the past year. Recently, several
variants such as B. 1.1. 7 (alpha), B. 1.351 (beta), and P. 1 (gamma), which share a key …

Single-molecule force spectroscopy is a unique method that can probe the structural
changes of single proteins at a high spatiotemporal resolution while mechanically …

Atomic force microscopy (AFM) based single-molecule force spectroscopy (SMFS) is a
powerful tool to study the mechanical properties of proteins. In these experiments, site …

Bacteriorhodopsin is a seven-helix light-driven proton-pump that was structurally and
functionally extensively studied. Despite a wealth of data, the single molecule kinetics of the …

Protein folding is a fundamental process of life with important implications throughout
biology. Indeed, tens of thousands of mutations have been associated with diseases, and …

Enzymes are widely used for protein ligation because of their efficient and site-specific
connections under mild conditions. However, many enzymatic ligations are restricted to …

Sortase is one of the most widely used enzymes for covalent protein conjugation that links
protein and protein/small molecules together in a site-specific way. It typically recognizes the …

Ligand-induced conformational changes are critical to the function of many membrane
proteins and arise from numerous intramolecular interactions. In the photocycle of the model …