Active transport between the nucleus and cytoplasm involves primarily three classes of
macromolecules: substrates, adaptors, and receptors. Some transport substrates bind …

Intense research in recent years has shown that many pre-mRNA processing events are co-
transcriptional or at least begin during RNA synthesis by RNA polymerase II (Pol II). But is it …

Transcriptome sequencing allows for analysis of mature RNAs at base pair resolution. Here
we show that RNA-seq can also be used for studying nascent RNAs undergoing …

To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced
nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty …

SR proteins are required for constitutive pre-mRNA splicing and also regulate alternative
splice site selection in a concentration-dependent manner. They have a modular structure …

Coupling between transcription and RNA processing is a key gene regulatory mechanism.
Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation …

Expression of most RNA polymerase II transcripts requires the coordinated execution of
transcription, splicing, and 3′ processing. We have previously shown that upon …

In vertebrates, a nuclear cap-binding complex (CBC) formed by two cap-binding proteins,
CBP20 and CBP80, is involved in several steps of RNA metabolism, including pre-mRNA …

To investigate the mechanism of spliceosome assembly in vivo, we performed chromatin
immunoprecipitation (ChIP) analysis of U1, U2, and U5 small nuclear ribonucleoprotein …

The pathways of synthesis and maturation of pre‐messenger RNA in the nucleus have a
direct effect on the translational efficiency of mRNA in the cytoplasm. The transcription of …