Cellular senescence, first observed and defined using in vitro cell culture studies, is an
irreversible cell cycle arrest which can be triggered by a variety of factors. Emerging …

Advanced in vitro models of human skeletal muscle tissue are increasingly needed to model
complex developmental dynamics and disease mechanisms not recapitulated in animal …

The array of genome editing strategies based on targeted double-stranded DNA break
formation have recently been enriched through the introduction of transcription activator-like …

The human nervous system is a highly complex but organized organ. The foundation of its
complexity and organization is laid down during regional patterning of the neural tube, the …

Cellular senescence, a stress induced growth arrest of somatic cells, was first documented
in cell cultures over 40 years ago, however its physiological significance has only recently …

Background Investigations into both the pathophysiology and therapeutic targets in muscle
dystrophies have been hampered by the limited proliferative capacity of human myoblasts …

Cultured human myoblasts fail to immortalize following the introduction of telomerase. The
availability of an immortalization protocol for normal human myoblasts would allow one to …

Engineered sequence-specific nucleases and donor DNA templates can be customized to
edit mammalian genomes via the homologous recombination (HR) pathway. Here we report …

Abstract CRISPR/Cas9-derived RNA-guided nucleases (RGNs) are DNA targeting systems,
which are rapidly being harnessed for gene regulation and gene editing purposes in model …

β-Catenin, apart from playing a cell-adhesive role, is a key nuclear effector of Wnt signaling.
Based on activity assays in Drosophila, we generated mouse strains where the endogenous …