The role of metagenomics and next-generation sequencing in infectious disease diagnosis
S Miller, C Chiu - Clinical chemistry, 2022 - academic.oup.com
Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is
becoming increasingly available as a method to identify pathogens in cases of suspected …
becoming increasingly available as a method to identify pathogens in cases of suspected …
SARS-CoV-2 pandemic: a review of molecular diagnostic tools including sample collection and commercial response with associated advantages and limitations
The unprecedented global pandemic known as SARS-CoV-2 has exercised to its limits
nearly all aspects of modern viral diagnostics. In doing so, it has illuminated both the …
nearly all aspects of modern viral diagnostics. In doing so, it has illuminated both the …
Bat coronaviruses related to SARS-CoV-2 and infectious for human cells
S Temmam, K Vongphayloth, E Baquero, S Munier… - Nature, 2022 - nature.com
The animal reservoir of SARS-CoV-2 is unknown despite reports of SARS-CoV-2-related
viruses in Asian Rhinolophus bats,,–, including the closest virus from R. affinis, RaTG13 …
viruses in Asian Rhinolophus bats,,–, including the closest virus from R. affinis, RaTG13 …
Adeno-associated virus type 2 in US children with acute severe hepatitis
V Servellita, A Sotomayor Gonzalez, DM Lamson… - Nature, 2023 - nature.com
As of August 2022, clusters of acute severe hepatitis of unknown aetiology in children have
been reported from 35 countries, including the USA,. Previous studies have found human …
been reported from 35 countries, including the USA,. Previous studies have found human …
Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids
We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA
from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids …
from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids …
Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions
In less than nine months, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-
CoV-2) killed over a million people, including> 25,000 in New York City (NYC) alone. The …
CoV-2) killed over a million people, including> 25,000 in New York City (NYC) alone. The …
Genomic surveillance reveals multiple introductions of SARS-CoV-2 into Northern California
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) has spread globally, with> 365,000 cases in …
syndrome coronavirus 2 (SARS-CoV-2) has spread globally, with> 365,000 cases in …
Upper airway gene expression reveals suppressed immune responses to SARS-CoV-2 compared with other respiratory viruses
SARS-CoV-2 infection is characterized by peak viral load in the upper airway prior to or at
the time of symptom onset, an unusual feature that has enabled widespread transmission of …
the time of symptom onset, an unusual feature that has enabled widespread transmission of …
Next generation and other sequencing technologies in diagnostic microbiology and infectious diseases
EE Hilt, P Ferrieri - Genes, 2022 - mdpi.com
Next-generation sequencing (NGS) technologies have become increasingly available for
use in the clinical microbiology diagnostic environment. There are three main applications of …
use in the clinical microbiology diagnostic environment. There are three main applications of …
Metagenomic next-generation sequencing of nasopharyngeal specimens collected from confirmed and suspect COVID-19 patients
HH Mostafa, JA Fissel, B Fanelli, Y Bergman… - MBio, 2020 - Am Soc Microbiol
Metagenomic next-generation sequencing (mNGS) offers an agnostic approach for
emerging pathogen detection directly from clinical specimens. In contrast to targeted …
emerging pathogen detection directly from clinical specimens. In contrast to targeted …