Life in Multi-Extreme Environments: Brines, Osmotic and Hydrostatic Pressure─ A Physicochemical View
JM Knop, S Mukherjee, MW Jaworek… - Chemical …, 2022 - ACS Publications
Elucidating the details of the formation, stability, interactions, and reactivity of biomolecular
systems under extreme environmental conditions, including high salt concentrations in …
systems under extreme environmental conditions, including high salt concentrations in …
Effects of Crowding and Cosolutes on Biomolecular Function at Extreme Environmental Conditions
The extent of the effect of cellular crowding and cosolutes on the functioning of proteins and
cells is manifold and includes the stabilization of the biomolecular systems, the excluded …
cells is manifold and includes the stabilization of the biomolecular systems, the excluded …
Assessing the influence of betaine-based natural deep eutectic systems on horseradish peroxidase
NF Gajardo-Parra, L Meneses… - ACS Sustainable …, 2022 - ACS Publications
To validate the use of horseradish peroxidase (HRP) in natural deep eutectic systems
(NADES), five different betaine-based NADES were characterized in terms of water content …
(NADES), five different betaine-based NADES were characterized in terms of water content …
Formate dehydrogenase: recent developments for NADH and NADPH recycling in Biocatalysis
A Maier, LM Mguni, ACR Ngo, D Tischler - ChemCatChem, 2024 - Wiley Online Library
Formate dehydrogenases (FDHs) catalyze the oxidation of formate to CO2 while reducing
NAD (P)+ to NAD (P) H and are classified into two main classes: metal‐dependent (Mo‐or W …
NAD (P)+ to NAD (P) H and are classified into two main classes: metal‐dependent (Mo‐or W …
Improving the separation of guaiacol from n-hexane by adding choline chloride to glycol extracting agents
Bio-oil is an important candidate to replace oil-derived products since it origins from
renewable sources such as biomass. However, oxygenated bio-oil-based compounds …
renewable sources such as biomass. However, oxygenated bio-oil-based compounds …
Leveraging liquid-liquid phase separation and volume modulation to regulate the enzymatic activity of formate dehydrogenase
L Ostermeier, M Ascani, N Gajardo-Parra… - Biophysical …, 2024 - Elsevier
Engineering of reaction media is an exciting alternative for modulating kinetic properties of
biocatalytic reactions. We addressed the combined effect of an aqueous two-phase system …
biocatalytic reactions. We addressed the combined effect of an aqueous two-phase system …
Natural Deep Eutectic Solvents Enhanced Electro-Enzymatic Conversion of CO2 to Methanol
Z Zhang, H Wang, Y Nie, X Zhang, X Ji - Frontiers in Chemistry, 2022 - frontiersin.org
Electro-enzymatic conversion of CO2 offers a promising solution for CO2 utilization, while
the conversion rate and efficiency were disappointing. To address the challenge, four kinds …
the conversion rate and efficiency were disappointing. To address the challenge, four kinds …
Osmolyte effect on enzymatic stability and reaction equilibrium of formate dehydrogenase
NF Gajardo-Parra, H Akrofi-Mantey, M Ascani… - Physical Chemistry …, 2022 - pubs.rsc.org
Osmolytes are well-known biocatalyst stabilisers as they promote the folded state of
proteins, and a stabilised biocatalyst might also improve reaction kinetics. In this work, the …
proteins, and a stabilised biocatalyst might also improve reaction kinetics. In this work, the …
Separation of furfuryl alcohol from water using hydrophobic deep eutectic solvents
E Cea-Klapp, AF Arroyo-Avirama… - Journal of Molecular …, 2023 - Elsevier
Furfuryl alcohol (FA) is an important organic chemical feedstock that must be separated from
water to upgrade it into high-value-added products. Since FA forms an azeotrope with water …
water to upgrade it into high-value-added products. Since FA forms an azeotrope with water …
Impact of Deep Eutectic Solvents on Kinetics and Folding Stability of Formate Dehydrogenase
NF Gajardo-Parra, G Rodríguez, AF Arroyo-Avirama… - Processes, 2023 - mdpi.com
Specifically designed co-solvent mixtures are an efficient way to enhance the kinetics of
enzyme-catalyzed reactions without compromising enzyme stability; among them, several …
enzyme-catalyzed reactions without compromising enzyme stability; among them, several …