Fusion proteins play an essential role in the biosciences but suffer from several key
limitations, including the requirement for N-to-C terminal ligation, incompatibility of …

Crosslinking of proteins has gained immense significance in the fabrication of biomaterials
for various health care applications. Various novel chemical-based strategies are being …

Protein–carbohydrate interactions play important roles in various biological processes, such
as organism development, cancer metastasis, pathogen infection and immune response, but …

Spatiotemporally resolved dissection of subcellular proteome is crucial to our understanding
of cellular functions in health and disease. We herein report a bioorthogonal and …

Proteins are multifunctional large organic compounds that constitute an essential
component of a living system. Hence, control over their bioconjugation impacts science at …

Cellular senescence, a process that arrests the cell cycle, is a cellular response mechanism
for various stresses and is implicated in aging and various age-related diseases. However …

Conspectus Despite advances in genome sequencing technology, the complete molecular
interaction networks reflecting the biological functions of gene products have not been fully …

Few chemical methods exist for the covalent conjugation of two proteins. We report the
preparation of site-specific protein–protein conjugates that arise from the sequential cross …

Spatially resolved in situ tagging of the cell of interest is crucial for in-depth mechanistic
dissection of multicellular architectures or processes. With continuing interest in …

In situ profiling of subcellular proteomics in primary living systems, such as native tissues or
clinic samples, is crucial for understanding life processes and diseases, yet challenging due …