Preparation of mixed-mode stationary phase for separation of peptides and proteins in high performance liquid chromatography

S Alharthi, A Ali, M Iqbal, A Ibrar, B Ahmad, S Nisa… - Scientific Reports, 2022 - nature.com
Porous silica particles were prepared by sol–gel method with some modification to get wide-
pore particles. These particles were derivatized with N-phenylmaleimide …

Hydroxypropyl‐β‐cyclodextrin encapsulated stationary phase based on silica monolith particles for enantioseparation in liquid chromatography

Y Lu, G Sun - Journal of Separation Science, 2021 - Wiley Online Library
Hydroxypropyl‐β‐cyclodextrin‐encapsulated stationary phase incorporated on silica
monolith particles was prepared by physical embedding, providing a new method for the …

Sedimentation assisted preparation of ground particles of silica monolith and their C18 modification resulting in a chromatographic phase of improved separation …

A Ali, F Ali, WJ Cheong - Journal of Chromatography A, 2017 - Elsevier
The sedimentation procedure has been adopted in production of ground silica monolith
particles to improve chromatographic separation efficiency of the resultant phase. First, silica …

Polystyrene bound silica monolith particles of reduced size as stationary phase of excellent separation efficiency in high performance liquid chromatograhy

A Ali, G Sun, JS Kim, WJ Cheong - Journal of Chromatography A, 2019 - Elsevier
Ground silica monolith particles of quite smaller average size (2 μm) have been prepared by
sol-gel process followed by soft grinding and calcination. Next a highly efficient …

Mixed‐mode open tubular column for peptide separations by capillary electrochromatography

F Ali, ZA AlOthman… - Journal of Separation …, 2021 - Wiley Online Library
Mixed‐mode chromatography open tubular column has been developed for peptide
separation in electrochromatography. A column with 92 cm effective length and 50 μm …

Open tubular capillary column for the separation of cytochrome C tryptic digest in capillary electrochromatography

F Ali, WJ Cheong - Journal of separation science, 2015 - Wiley Online Library
A silica capillary of 50 μm internal diameter and 500 mm length (416 mm effective length)
was chemically modified with 4‐(trifluoromethoxy) phenyl isocyanate in the presence of …

Particle packed mixed‐mode chromatographic stationary phase for the separation of peptide in liquid chromatography

F Ali, WJ Cheong, A Rafique… - Journal of …, 2021 - Wiley Online Library
A particle‐based stationary phase has been prepared for the separation of five synthetic
peptides and a mixture containing tryptic digest of cytochrome C in liquid chromatography …

Open tubular capillary electrochromatography with an N‐phenylacrylamide‐styrene copolymer‐based stationary phase for the separation of anomers of glucose and …

F Ali, WJ Cheong - Journal of Separation Science, 2015 - Wiley Online Library
A ligand with a terminal halogen (4‐chloromethylphenyl isocyanate) was chemically bound
on the inner surface of pretreated silica capillary with 50 μm internal diameter and 58 cm …

C18‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance

F Ali, WJ Cheong - Journal of separation science, 2014 - Wiley Online Library
Ground porous silica monolith particles with an average particle size of 2.34 μm and large
pores (363 Å) exhibiting excellent chromatographic performance have been synthesized on …

Styrene‐N‐phenylacrylamide co‐polymer modified silica monolith particles with an optimized mixing ratio of monomers as a new stationary phase for the separation …

G Sun, JS Kim, YS Kim, HJ An… - Journal of separation …, 2019 - Wiley Online Library
A stationary phase was prepared by chemical derivatization of the support particles with a
layer of copolymer composed of styrene and N‐phenyl acrylamide. Silica monolith particles …