Less label, more free: approaches in label‐free quantitative mass spectrometry

KA Neilson, NA Ali, S Muralidharan, M Mirzaei… - …, 2011 - Wiley Online Library
In this review we examine techniques, software, and statistical analyses used in label‐free
quantitative proteomics studies for area under the curve and spectral counting approaches …

Global and site‐specific analysis of protein glycosylation in complex biological systems with Mass Spectrometry

H Xiao, F Sun, S Suttapitugsakul… - Mass spectrometry …, 2019 - Wiley Online Library
Protein glycosylation is ubiquitous in biological systems and plays essential roles in many
cellular events. Global and site‐specific analysis of glycoproteins in complex biological …

Structural analysis of N-and O-glycans released from glycoproteins

PH Jensen, NG Karlsson, D Kolarich, NH Packer - Nature protocols, 2012 - nature.com
This protocol shows how to obtain a detailed glycan compositional and structural profile from
purified glycoproteins or protein mixtures, and it can be used to distinguish different isobaric …

DNA methylome and transcriptome alterations and cancer prevention by curcumin in colitis-accelerated colon cancer in mice

Y Guo, R Wu, JM Gaspar, D Sargsyan, ZY Su… - …, 2018 - academic.oup.com
Inflammation is highly associated with colon carcinogenesis. Epigenetic mechanisms could
play an important role in the initiation and progression of colon cancer. Curcumin, a dietary …

Heparan sulfate proteoglycan signaling in tumor microenvironment

V De Pasquale, LM Pavone - International Journal of Molecular Sciences, 2020 - mdpi.com
In the last few decades, heparan sulfate (HS) proteoglycans (HSPGs) have been an
intriguing subject of study for their complex structural characteristics, their finely regulated …

Comparative structural analysis of the glycosylation of salivary and buccal cell proteins: innate protection against infection by Candida albicans

AV Everest-Dass, D Jin, M Thaysen-Andersen… - …, 2012 - academic.oup.com
Mucosal epithelial surfaces, such as line the oral cavity, are common sites of microbial
colonization by bacteria, yeast and fungi. The microbial interactions involve adherence …

Glycomics and glycoproteomics of membrane proteins and cell‐surface receptors: Present trends and future opportunities

KB Chandler, CE Costello - Electrophoresis, 2016 - Wiley Online Library
Membrane proteins mediate cell–cell interactions and adhesion, the transfer of ions and
metabolites, and the transmission of signals from the extracellular environment to the cell …

NEGATIVE ION MASS SPECTROMETRY FOR THE ANALYSIS OF N‐LINKED GLYCANS

DJ Harvey - Mass Spectrometry Reviews, 2020 - Wiley Online Library
N‐glycans from glycoproteins are complex, branched structures whose structural
determination presents many analytical problems. Mass spectrometry, usually conducted in …

Structural analysis of glycoprotein sialylation–part II: LC-MS based detection

G Palmisano, MR Larsen, NH Packer… - Rsc Advances, 2013 - pubs.rsc.org
Structural characterisation of sialylated glycoproteins in time and space is a requirement for
further understanding of their involvement in biology. In this second of two related reviews …

Relative quantification of N-glycopeptide sialic acid linkage isomers by ion mobility mass spectrometry

X Feng, H Shu, S Zhang, Y Peng, L Zhang… - Analytical …, 2021 - ACS Publications
Sialic acids decorate the surface of glycoproteins and play important roles in a variety of
pathological processes. Although the mass spectrometry (MS) based linkage-specific …