The Flp Recombinase of th 2-μm Plasmid of Saccharomyces cerevisiae

PD Sadowski - Progress in nucleic acid research and molecular …, 1995 - Elsevier
Publisher Summary Most strains of the yeast, Saccharomyces cerevisiae, harbor about 100
copies of an autonomously replicating plasmid, the 2-μm plasmid. Although the plasmid …

[PDF][PDF] Site-specific recombinases: changing partners and doing the twist

P Sadowski - Journal of bacteriology, 1986 - Am Soc Microbiol
In hope of discovering universal biochemical mechanisms underlying these important
processes, several groups have followed the lead of Nash and colleagues (53) and have …

Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription

JA Doudna, M Jinek, E Charpentier… - US Patent …, 2019 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49624232&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription

JA Doudna, M Jinek, K Chylinski… - US Patent …, 2018 - Google Patents
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence
and, together with a modifying polypeptide, provides for site-specific modification of a target …

Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination

G Lee, I Saito - Gene, 1998 - Elsevier
The Cre recombinase mediates precise site-specific recombination between a pair of loxP
sequences through an intermediate containing Holiday junction. The recombination junction …

The integrase family of site‐specific recombinases: regional similarities and global diversity.

P Argos, A Landy, K Abremski, JB Egan… - The EMBO …, 1986 - embopress.org
A combination of two methods for detecting distant relationships in protein primary
sequences was used to compare the site‐specific recombination proteins encoded by …

Multiple new site-specific recombinases for use in manipulating animal genomes

A Nern, BD Pfeiffer, K Svoboda… - Proceedings of the …, 2011 - National Acad Sciences
Site-specific recombinases have been used for two decades to manipulate the structure of
animal genomes in highly predictable ways and have become major research tools …

Recombinational cloning using engineered recombination sites

JL Hartley, MA Brasch - US Patent 6,720,140, 2004 - Google Patents
Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in
vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered …

Identification of the Crossover Site During FLP-Mediated Recombination in the Saccharomyces cerevisiae Plasmid 2µm Circle

M McLeod, S Craft, JR Broach - Molecular and cellular biology, 1986 - Taylor & Francis
The FLP protein of the Saccharomyces cerevisiae plasmid 2 µm circle catalyzes site-specific
recombination between two repeated segments present on the plasmid. In this paper we …

Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription

JA Doudna, M Jinek, E Charpentier… - US Patent …, 2018 - Google Patents
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence
and, together with a modifying polypeptide, provides for site-specific modification of a target …