Expansions of simple tandem repeats are responsible for almost 50 human diseases, the
majority of which are severe, degenerative, and not currently treatable or preventable. In this …

The mechanisms that maintain the stability of chromosome ends have broad impact on
genome integrity in all eukaryotes. Budding yeast is a premier organism for telomere …

The replication of the ends of linear chromosomes, or telomeres, poses unique problems,
which must be solved to maintain genome integrity and to allow cell division to occur. Here …

First discovered as a structure-specific endonuclease that evolved to cut at the base of
single-stranded flaps, flap endonuclease (FEN1) is now recognized as a central component …

▪ Abstract Telomeres are DNA and protein structures that form complexes protecting the
ends of chromosomes. Understanding of the mechanisms maintaining telomeres and …

▪ Abstract One strand of cellular DNA is generated as RNA-initiated discontinuous segments
called Okazaki fragments that later are joined. The RNA terminated region is displaced into …

To better understand the requirements for telomerase-mediated telomere addition in vivo,
we developed an assay in S. cerevisiae that creates a chromosome end immediately …

DNA2 nuclease/helicase is a structure-specific nuclease, 5′-to-3′ helicase, and DNA-
dependent ATPase. It is involved in multiple DNA metabolic pathways, including Okazaki …

Telomeres protect chromosome ends from being detected as lesions and from triggering
DNA damage checkpoints. Paradoxically, telomere function depends on checkpoint proteins …

Abstract Flap endonuclease-1 (FEN1) is a member of the Rad2 structure-specific nuclease
family. FEN1 possesses FEN, 5′-exonuclease and gap-endonuclease activities. The …