In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR
associated protein (Cas) system, protoplasts are not only useful for rapidly validating the …

Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …

Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research
and breeding. Development of a foreign DNA-free clustered regularly interspaced short …

CRISPR/Cas-based genome editing technologies have the potential to fast-track large-scale
crop breeding programs. However, the rigid cell wall limits the delivery of CRISPR/Cas …

Plant male sterility (MS) represents the inability of the plant to generate functional anthers,
pollen, or male gametes. Developing MS lines represents one of the most important …

Chicory (Cichorium intybus var. sativum) is an industrial crop species cultivated for the
production of a fructose polymer inulin, which is used as a low‐calorie sweetener and …

Plant species have evolved diverse metabolic pathways to effectively respond to internal
and external signals throughout their life cycle, allowing adaptation to their sessile and …

Chicory taproots accumulate sesquiterpene lactones lactucin, lactucopicrin, and 8-
deoxylactucin, predominantly in their oxalated forms. The biosynthetic pathway for chicory …

Key message We have developed and validated an efficient protocol for producing gene-
edited carrot plants that do not result in the stable incorporation of foreign DNA in the edited …

Industrial chicory (Cichorium intybus var. sativum) and witloof (C. intybus var. foliosum) are
crops with an important economic value, mainly cultivated for inulin production and as a …