Protoplasts: from isolation to CRISPR/Cas genome editing application
JJ Yue, JL Yuan, FH Wu, YH Yuan… - Frontiers in Genome …, 2021 - frontiersin.org
In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR
associated protein (Cas) system, protoplasts are not only useful for rapidly validating the …
associated protein (Cas) system, protoplasts are not only useful for rapidly validating the …
Plant protoplasts in the age of synthetic biology
I Reyna-Llorens, M Ferro-Costa… - Journal of experimental …, 2023 - academic.oup.com
Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …
in plant research and have been instrumental in genetic transformation and the study of …
DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration
Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research
and breeding. Development of a foreign DNA-free clustered regularly interspaced short …
and breeding. Development of a foreign DNA-free clustered regularly interspaced short …
Non-GM genome editing approaches in crops
Z Gong, M Cheng, JR Botella - Frontiers in genome editing, 2021 - frontiersin.org
CRISPR/Cas-based genome editing technologies have the potential to fast-track large-scale
crop breeding programs. However, the rigid cell wall limits the delivery of CRISPR/Cas …
crop breeding programs. However, the rigid cell wall limits the delivery of CRISPR/Cas …
Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications
S Farinati, S Draga, A Betto, F Palumbo… - Frontiers in Plant …, 2023 - frontiersin.org
Plant male sterility (MS) represents the inability of the plant to generate functional anthers,
pollen, or male gametes. Developing MS lines represents one of the most important …
pollen, or male gametes. Developing MS lines represents one of the most important …
Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus L.
K Cankar, P Bundock, R Sevenier… - Plant Biotechnology …, 2021 - Wiley Online Library
Chicory (Cichorium intybus var. sativum) is an industrial crop species cultivated for the
production of a fructose polymer inulin, which is used as a low‐calorie sweetener and …
production of a fructose polymer inulin, which is used as a low‐calorie sweetener and …
Unlocking secrets of nature's chemists: Potential of CRISPR/Cas-based tools in plant metabolic engineering for customized nutraceutical and medicinal profiles
Plant species have evolved diverse metabolic pathways to effectively respond to internal
and external signals throughout their life cycle, allowing adaptation to their sessile and …
and external signals throughout their life cycle, allowing adaptation to their sessile and …
CRISPR/Cas9 targeted inactivation of the kauniolide synthase in chicory results in accumulation of costunolide and its conjugates in taproots
K Cankar, JC Hakkert, R Sevenier, E Campo… - Frontiers in Plant …, 2022 - frontiersin.org
Chicory taproots accumulate sesquiterpene lactones lactucin, lactucopicrin, and 8-
deoxylactucin, predominantly in their oxalated forms. The biosynthetic pathway for chicory …
deoxylactucin, predominantly in their oxalated forms. The biosynthetic pathway for chicory …
Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation
Key message We have developed and validated an efficient protocol for producing gene-
edited carrot plants that do not result in the stable incorporation of foreign DNA in the edited …
edited carrot plants that do not result in the stable incorporation of foreign DNA in the edited …
Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus
C De Bruyn, T Ruttink, E Lacchini… - Frontiers in Plant …, 2023 - frontiersin.org
Industrial chicory (Cichorium intybus var. sativum) and witloof (C. intybus var. foliosum) are
crops with an important economic value, mainly cultivated for inulin production and as a …
crops with an important economic value, mainly cultivated for inulin production and as a …