Allosteric cascade of spliceosome activation

DA Brow - Annual review of genetics, 2002 - annualreviews.org
▪ Abstract Introns are removed from precursor messenger RNAs in the cell nucleus by a
large ribonucleoprotein complex called the spliceosome. The spliceosome contains five …

Nucleic acid catalysis: metals, nucleobases, and other cofactors

WL Ward, K Plakos, VJ DeRose - Chemical reviews, 2014 - ACS Publications
The discovery of RNA-catalyzed phosphodiester bond cleavage by Cech and Altman in
1982− 1983 shattered the paradigm of protein-dependent biological catalysis and opened …

An atomic structure of the human spliceosome

X Zhang, C Yan, J Hang, LI Finci, J Lei, Y Shi - Cell, 2017 - cell.com
Mechanistic understanding of pre-mRNA splicing requires detailed structural information on
various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) …

Structure of a yeast activated spliceosome at 3.5 Å resolution

C Yan, R Wan, R Bai, G Huang, Y Shi - Science, 2016 - science.org
Pre–messenger RNA (pre-mRNA) splicing is carried out by the spliceosome, which
undergoes an intricate assembly and activation process. Here, we report an atomic structure …

RNA catalyses nuclear pre-mRNA splicing

SM Fica, N Tuttle, T Novak, NS Li, J Lu, P Koodathingal… - Nature, 2013 - nature.com
In nuclear pre-messenger RNA splicing, introns are excised by the spliceosome, a dynamic
machine composed of both proteins and small nuclear RNAs (snRNAs). Over thirty years …

Structure of a yeast spliceosome at 3.6-angstrom resolution

C Yan, J Hang, R Wan, M Huang, CCL Wong, Y Shi - Science, 2015 - science.org
Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome,
which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex) …

Structural basis of pre-mRNA splicing

J Hang, R Wan, C Yan, Y Shi - Science, 2015 - science.org
Splicing of precursor messenger RNA is performed by the spliceosome. In the cryogenic
electron microscopy structure of the yeast spliceosome, U5 small nuclear ribonucleoprotein …

Profiling lariat intermediates reveals genetic determinants of early and late co-transcriptional splicing

Y Zeng, BJ Fair, H Zeng, A Krishnamohan, Y Hou… - Molecular cell, 2022 - cell.com
Long introns with short exons in vertebrate genes are thought to require spliceosome
assembly across exons (exon definition), rather than introns, thereby requiring transcription …

Structure of a yeast catalytic step I spliceosome at 3.4 Å resolution

R Wan, C Yan, R Bai, G Huang, Y Shi - Science, 2016 - science.org
Each cycle of pre–messenger RNA splicing, carried out by the spliceosome, comprises two
sequential transesterification reactions, which result in the removal of an intron and the …

The 3.8 Å structure of the U4/U6. U5 tri-snRNP: Insights into spliceosome assembly and catalysis

R Wan, C Yan, R Bai, L Wang, M Huang, CCL Wong… - Science, 2016 - science.org
Splicing of precursor messenger RNA is accomplished by a dynamic megacomplex known
as the spliceosome. Assembly of a functional spliceosome requires a preassembled U4/U6 …