The coordinated expression of the Hox gene family encoding transcription factors is critical
for proper embryonic development and patterning. Major efforts have thus been dedicated to …

Transcription factors (TFs) are present in all life forms and conserved across great
evolutionary distances in eukaryotes. From yeast to complex multicellular organisms, they …

Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive
endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome …

Genome organization is driven by forces affecting transcriptional state, but the relationship
between transcription and genome architecture remains unclear. Here, we identified the …

Chromatin is a barrier to the binding of many transcription factors. By contrast, pioneer
factors access nucleosomal targets and promote chromatin opening. Despite binding to …

Background Hox transcription factors specify segmental diversity along the anterior-posterior
body axis in metazoans. While the different Hox family members show clear functional …

Oxidative metabolism is the predominant energy source for aerobic muscle contraction in
adult animals. How the cellular and molecular components that support aerobic muscle …

Abstract CRISPR-interference (CRISPRi), a highly effective method for silencing genes in
mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one …

Micrococcal nuclease sequencing is the state-of-the-art method for determining chromatin
structure and nucleosome positioning. Data analysis is complex due to the AT-dependent …

Background Chromosomes are subdivided spatially to delimit long-range interactions into
topologically associating domains (TADs). TADs are often flanked by chromatin insulators …