Deconstructing the chlamydial cell wall
A Klöckner, H Bühl, P Viollier, B Henrichfreise - Biology of Chlamydia, 2018 - Springer
The evolutionary separated Gram-negative Chlamydiales show a biphasic life cycle and
replicate exclusively within eukaryotic host cells. Members of the genus Chlamydia are …
replicate exclusively within eukaryotic host cells. Members of the genus Chlamydia are …
An NlpC/P60 protein catalyzes a key step in peptidoglycan recycling at the intersection of energy recovery, cell division and immune evasion in the intracellular …
J Reuter, C Otten, N Jacquier, J Lee… - PLoS …, 2023 - journals.plos.org
The obligate intracellular Chlamydiaceae do not need to resist osmotic challenges and thus
lost their cell wall in the course of evolution. Nevertheless, these pathogens maintain a …
lost their cell wall in the course of evolution. Nevertheless, these pathogens maintain a …
AmiD Is a Novel Peptidoglycan Amidase in Wolbachia Endosymbionts of Drosophila melanogaster
M Wilmes, K Meier, A Schiefer, M Josten… - Frontiers in Cellular …, 2017 - frontiersin.org
Wolbachia endobacteria are obligate intracellular bacteria with a highly reduced genome
infecting many arthropod and filarial species, in which they manipulate arthropod …
infecting many arthropod and filarial species, in which they manipulate arthropod …
The MraY Inhibitor Muraymycin D2 and Its Derivatives Induce Enlarged Cells in Obligate Intracellular Chlamydia and Wolbachia and Break the Persistence Phenotype …
I Löckener, LV Behrmann, J Reuter, A Schiefer… - Antibiotics, 2024 - mdpi.com
Chlamydial infections and diseases caused by filarial nematodes are global health
concerns. However, treatment presents challenges due to treatment failures potentially …
concerns. However, treatment presents challenges due to treatment failures potentially …
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation
J Huo, G Zhang, L Wang, W Sun, L Jia, X Yang… - Protein expression and …, 2021 - Elsevier
The gene encoding the phage major capsid protein 10A was cloned into the prokaryotic
expression vector pET24a, and a 6XHis-tag was fused to the 3′-end of the 10A gene to …
expression vector pET24a, and a 6XHis-tag was fused to the 3′-end of the 10A gene to …
Physicochemical screening for chemical stabilizer of erythropoietin to prevent its aggregation
Recombinant protein aggregation is a problematic issue and can provoke immunological
response. The aim of this study was to analyze the stability of erythropoietin (EPO), as a …
response. The aim of this study was to analyze the stability of erythropoietin (EPO), as a …
Expanding the understanding of peptidoglycan turnover in Chlamydiae
J Reuter - 2023 - bonndoc.ulb.uni-bonn.de
The obligate intracellular chlamydiae evolved mechanisms to persist inside their host and to
evade the host immune system, making them intriguing and highly successful pathogens …
evade the host immune system, making them intriguing and highly successful pathogens …
Application of formulation principles to stability issues encountered during processing, manufacturing, and storage of drug substance and drug product protein …
S Vitharana, JM Stillahn, DS Katayama… - Journal of …, 2023 - Elsevier
The field of formulation and stabilization of protein therapeutics has become rather
extensive. However, most of the focus has been on stabilization of the final drug product …
extensive. However, most of the focus has been on stabilization of the final drug product …
Enhancement of the stability of Mycobacterium tuberculosis recombinant antigen expressed in Escherichia coli using cell lysis additives
HY Kang, SH Ha, T Yoo, HJ Park, JA Lee… - Protein Expression and …, 2019 - Elsevier
Abstract Background Mycobacteria tuberculosis (Mtb), the causative agent of tuberculosis, is
a slow-growing bacterium. Expression in Escherichia coli is a widely used method for large …
a slow-growing bacterium. Expression in Escherichia coli is a widely used method for large …
Identification of new molecular targets and antibiotics as novel strategies against filarial infections
KA Meier - 2019 - bonndoc.ulb.uni-bonn.de
Wolbachia are obligate intracellular bacteria found in many arthropods and some filarial
nematodes that are pathogenic in humans. The Wolbachia genomes are highly reduced, but …
nematodes that are pathogenic in humans. The Wolbachia genomes are highly reduced, but …