CRISPR–Cas9 structures and mechanisms
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
A decade of discovery: CRISPR functions and applications
R Barrangou, P Horvath - Nature microbiology, 2017 - nature.com
This year marks the tenth anniversary of the identification of the biological function of
CRISPR–Cas as adaptive immune systems in bacteria. In just a decade, the characterization …
CRISPR–Cas as adaptive immune systems in bacteria. In just a decade, the characterization …
Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation
To acquire the ability to recognize and destroy virus and plasmid invaders, prokaryotic
CRISPR–Cas systems capture fragments of DNA within the host CRISPR locus. Our results …
CRISPR–Cas systems capture fragments of DNA within the host CRISPR locus. Our results …
CRISPR–Cas adaptation: insights into the mechanism of action
G Amitai, R Sorek - Nature Reviews Microbiology, 2016 - nature.com
Since the first demonstration that CRISPR–Cas systems provide bacteria and archaea with
adaptive immunity against phages and plasmids, numerous studies have yielded key …
adaptive immunity against phages and plasmids, numerous studies have yielded key …
Degradation of phage transcripts by CRISPR-associated RNases enables type III CRISPR-Cas immunity
Summary Type III-A CRISPR-Cas systems defend prokaryotes against viral infection using
CRISPR RNA (crRNA)-guided nucleases that perform co-transcriptional cleavage of the viral …
CRISPR RNA (crRNA)-guided nucleases that perform co-transcriptional cleavage of the viral …
Foreign DNA capture during CRISPR–Cas adaptive immunity
Bacteria and archaea generate adaptive immunity against phages and plasmids by
integrating foreign DNA of specific 30–40-base-pair lengths into clustered regularly …
integrating foreign DNA of specific 30–40-base-pair lengths into clustered regularly …
Surveillance and processing of foreign DNA by the Escherichia coli CRISPR-Cas system
CRISPR-Cas adaptive immune systems protect bacteria and archaea against foreign
genetic elements. In Escherichia coli, Cascade (CRISPR-associated complex for antiviral …
genetic elements. In Escherichia coli, Cascade (CRISPR-associated complex for antiviral …
The structural biology of CRISPR-Cas systems
Highlights•High-resolution Cascade structures provide a detailed view of Type IE
surveillance complex.•A working model is proposed to better understand Cascade-mediated …
surveillance complex.•A working model is proposed to better understand Cascade-mediated …
CRISPR-Cas: new tools for genetic manipulations from bacterial immunity systems
W Jiang, LA Marraffini - Annual review of microbiology, 2015 - annualreviews.org
Prokaryotic CRISPR-Cas loci encode proteins that function as an adaptive immune system
against infectious viruses and plasmids. Immunity is mediated by Cas nucleases and small …
against infectious viruses and plasmids. Immunity is mediated by Cas nucleases and small …
Diversity of CRISPR-Cas immune systems and molecular machines
R Barrangou - Genome biology, 2015 - Springer
Bacterial adaptive immunity hinges on CRISPR-Cas systems that provide DNA-encoded,
RNA-mediated targeting of exogenous nucleic acids. A plethora of CRISPR molecular …
RNA-mediated targeting of exogenous nucleic acids. A plethora of CRISPR molecular …