Modulation of DNA double-strand break repair as a strategy to improve precise genome editing

U Ray, SC Raghavan - Oncogene, 2020 - nature.com
In the present day, it is possible to incorporate targeted mutations or replace a gene using
genome editing techniques such as customisable CRISPR/Cas9 system. Although induction …

Understanding the DNA double-strand break repair and its therapeutic implications

U Ray, SC Raghavan - DNA repair, 2021 - Elsevier
Repair of DNA double-strand breaks (DSBs) and its regulation are tightly integrated inside
cells. Homologous recombination, nonhomologous end joining and microhomology …

Identification and characterization of mercaptopyrimidine‐based small molecules as inhibitors of nonhomologous DNA end joining

U Ray, VK Gopinatha, S Sharma, L Goyary… - The FEBS …, 2023 - Wiley Online Library
Mercaptopyrimidine derivatives are heterocyclic compounds with potent biological activities
including antiproliferative, antibacterial, and anti‐inflammatory properties. The present study …

Identification and characterization of novel SCR7‐based small‐molecule inhibitor of DNA end‐joining, SCR130 and its relevance in cancer therapeutics

U Ray, SK Raul, VK Gopinatha, D Ghosh… - Molecular …, 2020 - Wiley Online Library
Targeting DNA repair with small‐molecule inhibitors is an attractive strategy for cancer
therapy. Majority of DNA double‐strand breaks in mammalian cells are repaired through …

SCR7, an inhibitor of NHEJ can sensitize tumor cells to ionization radiation

V Gopalakrishnan, S Sharma, U Ray… - Molecular …, 2021 - Wiley Online Library
Nonhomologous end joining (NHEJ), one of the major DNA double‐strand break repair
pathways, plays a significant role in cancer cell proliferation and resistance to radio and …

SCR7, a potent cancer therapeutic agent and a biochemical inhibitor of nonhomologous DNA end‐joining

M Manjunath, B Choudhary, SC Raghavan - Cancer Reports, 2021 - Wiley Online Library
Background DNA double‐strand breaks (DSBs) are harmful to the cell as it could lead to
genomic instability and cell death when left unrepaired. Homologous recombination and …

Small-molecule enhancers of CRISPR-induced homology-directed repair in gene therapy: A medicinal chemist's perspective

ABC Lee, MH Tan, CLL Chai - Drug Discovery Today, 2022 - Elsevier
Highlights•Small molecules can tip the balance in favor of the HDR pathway over the NHEJ
pathway.•Cell cycle inhibitors and HDAC inhibitors can indirectly enhance precise …

Inhibitors of DNA double-strand break repair at the crossroads of cancer therapy and genome editing

U Ray, SC Raghavan - Biochemical Pharmacology, 2020 - Elsevier
Conventional cancer treatment modalities such as radiation and chemotherapy, cause
cancer cell death by inducing DNA damage, particularly DNA strand breaks. Over the years …

[HTML][HTML] Epz015938 Inhibitor

V Gopalakrishnan, S Sharma, U Ray, M Manjunath… - epz015938inhibitor.com
Nonhomologous end joining (NHEJ), one of the major DNA double‐strand break repair
pathways, plays a significant role in cancer cell proliferation and resistance to radio and …

Hypomorphic mutations in human DNA ligase IV lead to compromised DNA binding efficiency, hydrophobicity and thermal stability

ER Maddi, SC Raghavan… - … Engineering, Design and …, 2021 - academic.oup.com
Studies have shown that Lig4 syndrome mutations in DNA ligase IV (LigIV) are
compromised in its function with residual level of double strand break ligation activity in vivo …