PROTACs: great opportunities for academia and industry (an update from 2020 to 2021)

M He, C Cao, Z Ni, Y Liu, P Song, S Hao, Y He… - … and Targeted Therapy, 2022 - nature.com
Abstract PROteolysis TArgeting Chimeras (PROTACs) technology is a new protein-
degradation strategy that has emerged in recent years. It uses bifunctional small molecules …

[HTML][HTML] Structural biology of CRISPR–Cas immunity and genome editing enzymes

JY Wang, P Pausch, JA Doudna - Nature Reviews Microbiology, 2022 - nature.com
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

P Fozouni, S Son, MD de León Derby, GJ Knott… - Cell, 2021 - cell.com
The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …

[HTML][HTML] Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

AV Anzalone, LW Koblan, DR Liu - Nature biotechnology, 2020 - nature.com
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

Base editing rescue of spinal muscular atrophy in cells and in mice

M Arbab, Z Matuszek, KM Kray, A Du, GA Newby… - Science, 2023 - science.org
Spinal muscular atrophy (SMA), the leading genetic cause of infant mortality, arises from
survival motor neuron (SMN) protein insufficiency resulting from SMN1 loss. Approved …

[HTML][HTML] Programmable RNA targeting with the single-protein CRISPR effector Cas7-11

A Özcan, R Krajeski, E Ioannidi, B Lee, A Gardner… - Nature, 2021 - nature.com
CRISPR–Cas interference is mediated by Cas effector nucleases that are either components
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …

[HTML][HTML] Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing

ML Leibowitz, S Papathanasiou, PA Doerfler… - Nature …, 2021 - nature.com
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …

CRISPR-CasΦ from huge phages is a hypercompact genome editor

P Pausch, B Al-Shayeb, E Bisom-Rapp, CA Tsuchida… - Science, 2020 - science.org
CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive
immunity against virus infection and plasmid transformation. We describe a minimal …

[HTML][HTML] Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

KS Makarova, YI Wolf, J Iranzo, SA Shmakov… - Nature Reviews …, 2020 - nature.com
The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …

[HTML][HTML] Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes

C Xu, Y Zhou, Q Xiao, B He, G Geng, Z Wang, B Cao… - Nature …, 2021 - nature.com
Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …