Ion mobility mass spectrometry (IM-MS) for structural biology: insights gained by measuring mass, charge, and collision cross section
E Christofi, P Barran - Chemical Reviews, 2023 - ACS Publications
The investigation of macromolecular biomolecules with ion mobility mass spectrometry (IM-
MS) techniques has provided substantial insights into the field of structural biology over the …
MS) techniques has provided substantial insights into the field of structural biology over the …
Instrumentation at the leading edge of proteomics
The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …
To 200,000 m/z and Beyond: Native Electron Capture Charge Reduction Mass Spectrometry Deconvolves Heterogeneous Signals in Large Biopharmaceutical …
Great progress has been made in the detection of large biomolecular analytes by native
mass spectrometry; however, characterizing highly heterogeneous samples remains …
mass spectrometry; however, characterizing highly heterogeneous samples remains …
Top-down protein analysis by tandem-trapped ion mobility spectrometry/mass spectrometry (tandem-TIMS/MS) coupled with ultraviolet photodissociation (UVPD) and …
“Top-down” proteomics analyzes intact proteins and identifies proteoforms by their intact
mass as well as the observed fragmentation pattern in tandem mass spectrometry (MS/MS) …
mass as well as the observed fragmentation pattern in tandem mass spectrometry (MS/MS) …
Mobility-Assisted Pseudo-MS3 Sequencing of Protein Ions
KA Graham, VJ Grisolia, NB Borotto - Journal of the American …, 2024 - ACS Publications
The sequencing of intact proteins within a mass spectrometer has many benefits but is
frequently limited by the fact that tandem mass spectrometry (MS/MS) techniques often …
frequently limited by the fact that tandem mass spectrometry (MS/MS) techniques often …
Combining SDS‐PAGE to capillary zone electrophoresis‐tandem mass spectrometry for high‐resolution top‐down proteomics analysis of intact histone proteoforms
Mass spectrometry (MS)‐based top‐down proteomics (TDP) analysis of histone proteoforms
provides critical information about combinatorial post‐translational modifications (PTMs) …
provides critical information about combinatorial post‐translational modifications (PTMs) …
Top/Middle-Down Characterization of α-Synuclein Glycoforms
SA Miller, K Jeanne Dit Fouque, ER Hard… - Analytical …, 2023 - ACS Publications
α-Synuclein is an intrinsically disordered protein that plays a critical role in the pathogenesis
of neurodegenerative disorders, such as Parkinson's disease. Proteomics studies of human …
of neurodegenerative disorders, such as Parkinson's disease. Proteomics studies of human …
Top-down proteomics
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
Capillary zone electrophoresis‐high field asymmetric ion mobility spectrometry‐tandem mass spectrometry for top‐down characterization of histone proteoforms
Abstract Characterization of histone proteoforms with various post‐translational
modifications (PTMs) is critical for a better understanding of functions of histone proteoforms …
modifications (PTMs) is critical for a better understanding of functions of histone proteoforms …
Conformational Characterization of Peptides and Proteins by 193 nm Ultraviolet Photodissociation in the Collision Cell of a Trapped Ion Mobility Spectrometry-Time-of …
Ultraviolet photodissociation (UVPD) has been shown to be a versatile ion activation
strategy for the characterization of peptides and intact proteins among other classes of …
strategy for the characterization of peptides and intact proteins among other classes of …