We have developed a robust platform to generate and functionally characterize rabbit-
derived antibodies using B cells from peripheral blood. The rapid high throughput procedure …

Abstract VDJ rearrangement and VH gene usage during fetal development in 35 outbred
piglets was examined by PCR amplification of VDJs; VDJs were subsequently characterized …

Since the actual combinatorial diversity in the VH repertoire in fetal piglets represents< 1%
of the potential in mice and humans, we wondered whether 1) complementarity-determining …

A bacterial artificial chromosome (BAC) library was created using partially digested rabbit
chromosomal DNA. Four BAC clones spanning about 0.5 Mb of the rabbit immunoglobulin …

We have used a bioinformatics approach to evaluate the completeness and functionality of
the reported human immunoglobulin heavy-chain IGHD gene repertoire. Using the hidden …

The young rabbit appendix and the chicken bursa of Fabricius are primary lymphoid organs
where the B cell Ab repertoire develops in germinal centers (GCs) mainly by a gene …

The young rabbit appendix is a dynamic site for primary B cell repertoire development. To
study diversification patterns during clonal expansion, we collected single appendix B cells …

We report the cloning and analysis of a bovine JH locus comprising a DQ52 segment, six JH
segments and sequence to a 5′ H chain intronic enhancer. The contig was mapped to BTA …

Circular DNA, derived from lymphocytes of juvenile channel catfish, was used to construct λ
libraries that were screened to identify the products of immunoglobulin D HJ H excision …

Vaccines represent one of the most impressive successes of experimental medicine,
allowing for the eradication of a causative infectious agent and extinction of diseases such …