Proteins are nature's primary building blocks for the construction of sophisticated molecular
machines and dynamic materials, ranging from protein complexes such as photosystem II …

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …

Deep learning generative approaches provide an opportunity to broadly explore protein
structure space beyond the sequences and structures of natural proteins. Here, we use deep …

Nature provides many examples of self-and co-assembling protein-based molecular
machines, including icosahedral protein cages that serve as scaffolds, enzymes, and …

Natural molecular machines contain protein components that undergo motion relative to
each other. Designing such mechanically constrained nanoscale protein architectures with …

The challenges of evolution in a complex biochemical environment, coupling genotype to
phenotype and protecting the genetic material, are solved elegantly in biological systems by …

We describe a procedure for designing proteins with backbones produced by varying the
parameters in the Crick coiled coil–generating equations. Combinatorial design calculations …

Asymmetric multiprotein complexes that undergo subunit exchange play central roles in
biology but present a challenge for design because the components must not only contain …

We describe a general computational approach to designing self-assembling helical
filaments from monomeric proteins and use this approach to design proteins that assemble …

The formation of large, well-ordered crystals for crystallographic experiments remains a
crucial bottleneck to the structural understanding of many important biological systems. To …