Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Recent progress in fluorescence imaging allows neuroscientists to observe the dynamics of
thousands of individual neurons, identified genetically or by their connectivity, across …

Brains contain networks of interconnected neurons and so knowing the network architecture
is essential for understanding brain function. We therefore mapped the synaptic-resolution …

The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

Current imaging approaches limit the ability to perform multi-scale characterization of three-
dimensional (3D) organotypic cultures (organoids) in large numbers. Here, we present an …

Light-field microscopy has emerged as a technique of choice for high-speed volumetric
imaging of fast biological processes. However, artifacts, nonuniform resolution and a slow …

Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular
behaviors and organelle functions during native physiological processes. However, optical …

By providing spatial, molecular and morphological data over time, live-cell imaging can
provide a deeper understanding of the cellular and signalling events that determine cancer …

The mesencephalic locomotor region (MLR) is a brain stem area whose stimulation triggers
graded forward locomotion. How MLR neurons recruit downstream vsx2+(V2a) …

Abstract High-speed three-dimensional (3D) intravital imaging in animals is useful for
studying transient subcellular interactions and functions in health and disease. Light-field …