Liquid chromatography is a technique that is gaining increasing interest and application, for
example in the pharmaceutical industry. At the same time as the increase in the number of …

The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild‐type
S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have …

Protein A, a major cell wall component of Staphylococcus aureus, is one of the first
immunoglobulin-binding proteins that is discovered about 80 years ago. However, a great …

Abstract Background Human Growth Hormone (hGH) is a glycoprotein released from the
pituitary gland. Due to the wide range of effects in humans, any disruption in hGH secretion …

The aim of the present work is to evaluate the possibilities and limitations of reversed
hydrophilic interaction chromatography (revHILIC) mode in liquid chromatography (LC). This …

The recombinant Staphylococcal protein A (SpA) is widely used in biotechnology to purify
polyclonal and monoclonal IgG antibodies. At very low concentrations, the highly-purified …

Hydrophilic column combined with mobile phase containing high content of water is a green
method for the separation of polar compounds, but there are few related studies, and the …

Abstract Background: The recombinant Staphylococcal protein A (SpA) is widely used in
biotechnology to purify polyclonal and monoclonal IgG antibodies. At very low …

In this work, a polar stationary phase was prepared by thermal immobilization of poly (
glycidoxypropylmethyl-co-dimethylsiloxane) onto silica particles to separate polar …

Poly (ethylene oxide-co-dimethylsiloxane) was thermally immobilized on silica particles-Si
(PEO)-to separate small polar compounds with water-rich mobile phases. Poly (ethylene …