Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Recent advances in ω-transaminase-mediated biocatalysis for the enantioselective synthesis of chiral amines

M D. Patil, G Grogan, A Bommarius, H Yun - Catalysts, 2018 - mdpi.com
Chiral amines are important components of 40–45% of small molecule pharmaceuticals and
many other industrially important fine chemicals and agrochemicals. Recent advances in …

Oxidoreductase-catalyzed synthesis of chiral amines

MD Patil, G Grogan, A Bommarius, H Yun - Acs Catalysis, 2018 - ACS Publications
Chiral amines are valuable constituents of many important pharmaceutical compounds and
their intermediates. It is estimated that∼ 40%–45% of small molecule pharmaceuticals …

Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater

J Guo, C Chen, W Chen, J Jiang, B Chen, F Zheng - Chemosphere, 2021 - Elsevier
To overcome the easy loss of microorganism, the mass production of sludge and the
consumption of aeration energy during biological treatment of anaerobically digested swine …

Pseudomonas putida KT2440 is HV1 certified, not GRAS

LFC Kampers, RJM Volkers… - Microbial …, 2019 - Wiley Online Library
Pseudomonas putida is rapidly becoming a workhorse for industrial production due to its
metabolic versatility, genetic accessibility and stress‐resistance properties. The P. putida …

Deracemization of Racemic Amines to Enantiopure (R)‐ and (S)‐amines by Biocatalytic Cascade Employing ω‐Transaminase and Amine Dehydrogenase

S Yoon, MD Patil, S Sarak, H Jeon, GH Kim… - …, 2019 - Wiley Online Library
A one‐pot deracemization strategy for α‐chiral amines is reported involving an
enantioselective deamination to the corresponding ketone followed by a stereoselective …

Enzymatic synthesis of sitagliptin intermediate using a novel ω-transaminase

GH Kim, H Jeon, TP Khobragade, MD Patil… - Enzyme and microbial …, 2019 - Elsevier
Enantiopure β-amino acids are essential precursors of various pharmaceuticals,
agrochemicals and other industrially important chemicals. In this study, we selected sixteen …

Cross-linked β-mannanase aggregates: preparation, characterization, and application for producing partially hydrolyzed guar gum

S Behera, MJ Dev, RS Singhal - Applied Biochemistry and Biotechnology, 2022 - Springer
Abstract β-Mannanase capable of hydrolyzing β-1, 4-linkages in guar gum was immobilized
as cross-linked enzyme aggregates (M-CLEAs). The aggregation and cross-linking process …

Parallel anti-sense two-step cascade for alcohol amination leading to ω-amino fatty acids and α, ω-diamines

S Sung, H Jeon, S Sarak, MM Ahsan, MD Patil… - Green …, 2018 - pubs.rsc.org
Running two two-step cascades in parallel anti-sense to transform an alcohol to an amine
allowed the conversion of ω-hydroxy fatty acids (ω-HFAs) and α, ω-diols to the …

[HTML][HTML] Metabolic engineering of Pseudomonas putida KT2440 for medium-chain-length fatty alcohol and ester production from fatty acids

C Lu, EO Akwafo, RH Wijffels, VAPM Dos Santos… - Metabolic …, 2023 - Elsevier
Medium-chain-length fatty alcohols have broad applications in the surfactant, lubricant, and
cosmetic industries. Their acetate esters are widely used as flavoring and fragrance …