Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

Nucleic acid cleavage processes contribute to target and signal amplification, which is
critical for the development of highly sensitive assays. 1, 2 Exonucleases or endonucleases …

Conventional nucleic acid detection technologies usually rely on amplification to improve
sensitivity, which has drawbacks, such as amplification bias, complicated operation, high …

Digital bioassays based on single-molecule enzyme reactions represent a new class of
bioanalytical methods that enable the highly sensitive detection of biomolecules in a …

CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

Quantification of HIV RNA in plasma is critical for identifying the disease progression and
monitoring the effectiveness of antiretroviral therapy. While RT-qPCR has been the gold …

The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …

The continuing evolution of the SARS-CoV-2 virus has led to the emergence of many
variants, including variants of concern (VOCs). CRISPR-Cas systems have been used to …

Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced
short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and …

Digital CRISPR (dCRISPR) assays are an emerging platform of molecular diagnostics.
Digital platforms introduce absolute quantification and increased sensitivity to bulk CRISPR …