Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9
SV Prykhozhij, C Fuller, SL Steele… - Nucleic acids …, 2018 - academic.oup.com
We have optimized point mutation knock-ins into zebrafish genomic sites using clustered
regularly interspaced palindromic repeats (CRISPR)/Cas9 reagents and single-stranded …
regularly interspaced palindromic repeats (CRISPR)/Cas9 reagents and single-stranded …
Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency
AA Ruparelia, V Oorschot, R Vaz, G Ramm… - Acta …, 2014 - Springer
Mutations in the co-chaperone Bcl2-associated athanogene 3 (BAG3) can cause myofibrillar
myopathy (MFM), a childhood-onset progressive muscle disease, characterized by the …
myopathy (MFM), a childhood-onset progressive muscle disease, characterized by the …
A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
SV Prykhozhij, SL Steele, B Razaghi… - Disease models & …, 2017 - journals.biologists.com
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive
immunity against pathogens in bacteria has been adapted for genome editing and applied …
immunity against pathogens in bacteria has been adapted for genome editing and applied …
FLNC myofibrillar myopathy results from impaired autophagy and protein insufficiency
AA Ruparelia, V Oorschot, G Ramm… - Human molecular …, 2016 - academic.oup.com
Myofibrillar myopathy is a progressive muscle disease characterized by the disintegration of
muscle fibers and formation of protein aggregates. Causative mutations have been identified …
muscle fibers and formation of protein aggregates. Causative mutations have been identified …
Characterisation of evolutionarily conserved key players affecting eukaryotic flagellar motility and fertility using a moss model
Defects in flagella/cilia are often associated with infertility and disease. Motile male gametes
(sperm cells) are an ancestral eukaryotic trait that has been lost in several lineages like …
(sperm cells) are an ancestral eukaryotic trait that has been lost in several lineages like …
[HTML][HTML] Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammation
CM Biggs, A Cordeiro-Santanach, SV Prykhozhij… - JCI insight, 2022 - ncbi.nlm.nih.gov
Primary atopic disorders are a group of inborn errors of immunity that skew the immune
system toward severe allergic disease. Defining the biology underlying these extreme …
system toward severe allergic disease. Defining the biology underlying these extreme …
In vivo imaging of protein interactions in the germplasm with bimolecular fluorescent complementation
RP Perera, R Dosch - Germline Development in the Zebrafish: Methods …, 2021 - Springer
Protein–protein interactions (PPIs) play a central role in all cellular processes. The discovery
of green fluorescent protein (GFP) and split varieties, which are functionally reconstituted by …
of green fluorescent protein (GFP) and split varieties, which are functionally reconstituted by …
Successful optimization of CRISPR/Cas9-mediated defined point mutation knock-in using allele-specific PCR assays in zebrafish
SV Prykhozhij, C Fuller, SL Steele, CJ Veinotte… - bioRxiv, 2017 - biorxiv.org
Single-stranded oligodeoxynucleotides (ssODN) are donor templates for homology-directed
repair-based knock-in of point mutations using CRISPR/Cas9. To optimize the efficiency of …
repair-based knock-in of point mutations using CRISPR/Cas9. To optimize the efficiency of …