Quantitative PCR (qPCR) is a widely-used technique for quantifying the expression of target
genes across various tissues, as well as under different pathological and physiological …

Normalization of gene expression data using appropriate reference genes is critical to
diminish any technical bias in an experiment involving quantitative real-time PCR (qPCR) …

The objective of the present study was to evaluate the stability of candidate reference genes
and select the genes that can be used for normalizing real-time polymerase chain reaction …

Accurate normalization of the gene expression assays, using housekeeping genes (HKGs),
is critically necessary. To do so, selection of a proper set of HKGs for a specific experiment is …

Environmental temperature is one of the major factors to affect health and productivity of
dairy cattle. Gene expression networks within the cells and tissues coordinate stress …

Background Quantitative real-time PCR (qPCR) is a highly reliable method for validating
gene expression data in molecular studies due to its sensitivity, specificity, and efficiency. To …

Despite the involvement of both immune cells and soluble factors in the etiopathogenesis of
cystic ovarian disease (COD), the precise interplay between these components in this …

Simple Summary Aquaporins are membrane channels that allow for the movement of water
and solutes in cells. They have been reported to play crucial roles in mammalian early …

Quantitative PCR (qPCR) is a highly sensitive, cost effective, and routinely used molecular
assay for analyzing the gene expression patterns of specific target genes across tissues …

Materials and Methods: Real-time polymerase chain reaction was performed to analyze the
transcript of hsp90, hsp70, hsp60, and hsf1 in the whole blood of Romosinuano under …