Quantitative data analysis in single-molecule localization microscopy
Super-resolution microscopy, and specifically single-molecule localization microscopy
(SMLM), is becoming a transformative technology for cell biology, as it allows the study of …
(SMLM), is becoming a transformative technology for cell biology, as it allows the study of …
PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing
E Chuntharpursat-Bon, OV Povstyan… - Communications …, 2023 - nature.com
Two prominent concepts for the sensing of shear stress by endothelium are the PIEZO1
channel as a mediator of mechanically activated calcium ion entry and the PECAM1 cell …
channel as a mediator of mechanically activated calcium ion entry and the PECAM1 cell …
Affimers and nanobodies as molecular probes and their applications in imaging
P Cordell, G Carrington, A Curd, F Parker… - Journal of Cell …, 2022 - journals.biologists.com
Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the
past five to ten years, many new labelling tools have been developed with significant …
past five to ten years, many new labelling tools have been developed with significant …
Maximum-likelihood model fitting for quantitative analysis of SMLM data
Quantitative data analysis is important for any single-molecule localization microscopy
(SMLM) workflow to extract biological insights from the coordinates of the single …
(SMLM) workflow to extract biological insights from the coordinates of the single …
Particle fusion of super-resolution data reveals the unit structure of Nup96 in Nuclear Pore Complex
Single molecule localization microscopy offers resolution nearly down to the molecular level
with specific molecular labelling, and is thereby a promising tool for structural biology. In …
with specific molecular labelling, and is thereby a promising tool for structural biology. In …
Assessment of 3D MINFLUX data for quantitative structural biology in cells
In 2017, Balzarotti et al. 1 introduced MINFLUX to localize individual fluorophores in single-
molecule localization microscopy by probing the emitter with a donut-shaped excitation …
molecule localization microscopy by probing the emitter with a donut-shaped excitation …
[PDF][PDF] 核孔复合物单分子定位超分辨图像的筛选和重构
侯梦迪， 胡芬， 杨建宇， 董浩， 潘雷霆 - 中国激光, 2024 - researching.cn
摘要核孔复合物(NPC) 是细胞核膜上由多种蛋白组装而成的复杂结构, 在细胞核质交换和信息
传递中起着关键作用. 单分子定位超分辨成像(SMLM) 以其特异性和高成像分辨率成为研究NPC …
传递中起着关键作用. 单分子定位超分辨成像(SMLM) 以其特异性和高成像分辨率成为研究NPC …
[HTML][HTML] Super-resolution imaging pinpoints the periodic ultrastructure at the human node of Ranvier and its disruption in patients with polyneuropathy
L Appeltshauser, J Linke, HS Heil, C Karus… - Neurobiology of …, 2023 - Elsevier
The node of Ranvier is the key element in saltatory conduction along myelinated axons, but
its specific protein organization remains elusive in the human species. To shed light on …
its specific protein organization remains elusive in the human species. To shed light on …
Raw data to results: a hands-on introduction and overview of computational analysis for single-molecule localization microscopy
Single-molecule localization microscopy (SMLM) is an advanced microscopy method that
uses the blinking of fluorescent molecules to determine the position of these molecules with …
uses the blinking of fluorescent molecules to determine the position of these molecules with …
A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types
ME Hurley, TMD Sheard, R Norman, HM Kirton… - Methods, 2021 - Elsevier
Nanometre-scale cellular information obtained through super-resolution microscopies are
often unaccompanied by functional information, particularly transient and diffusible signals …
often unaccompanied by functional information, particularly transient and diffusible signals …