One of the first biological machineries to be created seems to have been the ribosome.
Since then, organisms have dedicated great efforts to optimize this apparatus. The …

Simple Summary Building ribosomes for cellular protein translation is a massive, energy-
consuming undertaking. The process is executed by all cells to replenish the relevant pools …

Cancer cells are dependent upon an abundance of ribosomes to maintain rapid cell growth
and proliferation. The rate-limiting step of ribosome biogenesis is ribosomal RNA (rRNA) …

Eukaryotic RNA polymerase II (Pol II) is an essential enzyme that lies at the core of
eukaryotic biology. Due to its pivotal role in gene expression, Pol II has been subjected to a …

Abstract RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA), which is the first and
rate-limiting step in ribosome biosynthesis. A12. 2 (A12) is a critical subunit of Pol I that is …

Eukaryotes express three DNA-dependent RNA polymerases (Pols) that are responsible for
the entirety of cellular genomic expression. The three Pols have evolved to express specific …

Eukaryotes express three nuclear RNA polymerases (Pols I, II, and III) that are essential for
cell survival. Despite extensive investigation of the three Pols, significant knowledge gaps …

Here we present an optimized protocol for monitoring and analyzing single nucleotide
incorporation by RNA polymerases. This protocol describes the assembly of Saccharomyces …

Simple Summary Cancer cells upregulate RNA polymerase I (Pol I) activity to increase
ribosome abundance in support of rapid cell growth and proliferation. During the last …

Simple Summary Ribosomes are macromolecular complexes responsible for mRNA
translation. When ribosome biogenesis is hyperactive or aberrant or ribosomal factors are …