In biological systems, form and function are inherently correlated. Despite this strong
interdependence, the biological effect of curvature has been largely overlooked or …

Despite the interest in systemic delivery of therapeutic molecules including macromolecular
proteins and peptides via the lung, the accurate assessment of their pulmonary …

Inhaled siRNA therapy has a unique potential for treatment of severe lung diseases, such as
cystic fibrosis (CF). Nevertheless, a drug delivery system tackling lung barriers is mandatory …

Purpose The aim of this study was to compare the effect of liquid-covered culture (LCC) and
air-interfaced culture (AIC) on Calu-3 cell layer morphology and permeability, thus …

The aim of the current study was to evaluate primary (human bronchial epithelial cells,
HBEC) and non‐primary (Calu‐3, BEAS‐2B, BEAS‐2B R1) bronchial epithelial cell culture …

Oral inhalation results in pulmonary drug targeting and thereby reduces systemic side
effects, making it the preferred means of drug delivery for the treatment of respiratory …

In previous studies, we demonstrated that allergenic house dust mite proteases are potent
inducers of proinflammatory cytokines from the respiratory epithelium, although the precise …

Recent developments in delivering drugs to the lung are driving the need for in vitro
methods to evaluate the fate of inhaled medicines. Constraints on experimentation using …

In this study we have investigated the effects of different cell culture conditions on the Calu-3
epithelial cell model. Calu-3 cells were cultured in media A-MEM at the air–liquid (A–L) or …

Background Epithelial barrier dysfunction and increased permeability may contribute to
antigen sensitization and disease progression in asthma. Claudin-18.1 is the only known …