Single-molecule localization microscopy in eukaryotes

M Sauer, M Heilemann - Chemical reviews, 2017 - ACS Publications
Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Correlated light and electron microscopy: ultrastructure lights up!

P De Boer, JP Hoogenboom, BNG Giepmans - Nature methods, 2015 - nature.com
Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

DP Hoffman, G Shtengel, CS Xu, KR Campbell… - Science, 2020 - science.org
INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Super-resolution microscopy as a powerful tool to study complex synthetic materials

S Pujals, N Feiner-Gracia, P Delcanale, I Voets… - Nature Reviews …, 2019 - nature.com
Understanding the relations between the formation, structure, dynamics and functionality of
complex synthetic materials is one of the great challenges in chemistry and nanotechnology …

Focused ion beams in biology

K Narayan, S Subramaniam - Nature methods, 2015 - nature.com
A quiet revolution is under way in technologies used for nanoscale cellular imaging.
Focused ion beams, previously restricted to the materials sciences and semiconductor …

Correlative super-resolution microscopy: new dimensions and new opportunities

M Hauser, M Wojcik, D Kim, M Mahmoudi, W Li… - Chemical …, 2017 - ACS Publications
Correlative microscopy, the integration of two or more microscopy techniques performed on
the same sample, produces results that emphasize the strengths of each technique while …

Super-resolving microscopy in neuroscience

C Werner, M Sauer, C Geis - Chemical Reviews, 2021 - ACS Publications
Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …

Whole-cell, 3D, and multicolor STED imaging with exchangeable fluorophores

C Spahn, JB Grimm, LD Lavis, M Lampe… - Nano …, 2018 - ACS Publications
We demonstrate stimulated emission depletion (STED) microscopy of whole bacterial and
eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A …

From single molecules to life: microscopy at the nanoscale

B Turkowyd, D Virant, U Endesfelder - Analytical and bioanalytical …, 2016 - Springer
Super-resolution microscopy is the term commonly given to fluorescence microscopy
techniques with resolutions that are not limited by the diffraction of light. Since their …

Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy

V Liss, B Barlag, M Nietschke, M Hensel - Scientific reports, 2015 - nature.com
Research in cell biology demands advanced microscopy techniques such as confocal
fluorescence microscopy (FM), super-resolution microscopy (SRM) and transmission …