[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

Physiological barriers and strategies of lipid‐based nanoparticles for nucleic acid drug delivery

M Hu, X Li, Z You, R Cai, C Chen - Advanced Materials, 2024 - Wiley Online Library
Lipid‐based nanoparticles (LBNPs) are currently the most promising vehicles for nucleic
acid drug (NAD) delivery. Although their clinical applications have achieved success, the …

Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing

X Tang, Y Zhang - New Phytologist, 2023 - Wiley Online Library
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …

Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

Z Kocsisova, V Coneva - Frontiers in Genome Editing, 2023 - frontiersin.org
Increased understanding of plant genetics and the development of powerful and easier-to-
use gene editing tools over the past century have revolutionized humankind's ability to …

Crop bioengineering via gene editing: reshaping the future of agriculture

M Atia, W Jiang, K Sedeek, H Butt, M Mahfouz - Plant Cell Reports, 2024 - Springer
Genome-editing technologies have revolutionized research in plant biology, with major
implications for agriculture and worldwide food security, particularly in the face of challenges …

Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis

OS Adeyinka, B Tabassum, BL Koloko, IV Ogungbe - Planta, 2023 - Springer
Main conclusion The enhancement of CRISPR-Cas gene editing with robust nuclease
activity promotes genetic modification of desirable agronomic traits, such as resistance to …

Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava

D Tuo, Y Yao, P Yan, X Chen, F Qu, W Xue, J Liu… - Plant Methods, 2023 - Springer
Background Plant virus vectors designed for virus-mediated protein overexpression (VOX),
virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost …

CRISPR beyond: harnessing compact RNA-guided endonucleases for enhanced genome editing

F Wang, S Ma, S Zhang, Q Ji, C Hu - Science China Life Sciences, 2024 - Springer
The CRISPR-Cas system, an adaptive immunity system in prokaryotes designed to combat
phages and foreign nucleic acids, has evolved into a groundbreaking technology enabling …

CRISPR/Cas-mediated in planta gene targeting: current advances and challenges

I Guzmán-Benito, NP Achkar… - Journal of …, 2023 - academic.oup.com
Gene targeting can be used to make modifications at a specific region in a plant's genome
and create high-precision tools for plant biotechnology and breeding. However, its low …

Engineer and split an efficient hypercompact CRISPR–CasΦ genome editor in plants

Y Sun, J Hu, Z Hu, H Zhou, Y Gao, Y Liu, Y Ji, G Xu… - Plant …, 2024 - cell.com
The programmable CRISPR-Cas genome editing technology, adopted from prokaryotic
adaptive immune systems, has revolutionized genome engineering in plants (Liu et al …