CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …
Precision genome editing using cytosine and adenine base editors in mammalian cells
Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …
treating genetic diseases. Our laboratory developed base editing to enable precise and …
The design and application of DNA-editing enzymes as base editors
KL Rallapalli, AC Komor - Annual review of biochemistry, 2023 - annualreviews.org
DNA-editing enzymes perform chemical reactions on DNA nucleobases. These reactions
can change the genetic identity of the modified base or modulate gene expression. Interest …
can change the genetic identity of the modified base or modulate gene expression. Interest …
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
MG Kluesner, WS Lahr, C Lonetree… - Nature …, 2021 - nature.com
CRISPR-Cas9 cytidine and adenosine base editors (CBEs and ABEs) can disrupt genes
without introducing double-stranded breaks by inactivating splice sites (BE-splice) or by …
without introducing double-stranded breaks by inactivating splice sites (BE-splice) or by …
Enrichment strategies to enhance genome editing
NS Mikkelsen, RO Bak - Journal of Biomedical Science, 2023 - Springer
Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …
understanding of cellular and disease mechanisms and the development of gene and …
Structure-guided inhibition of the cancer DNA-mutating enzyme APOBEC3A
The normally antiviral enzyme APOBEC3A is an endogenous mutagen in human cancer. Its
single-stranded DNA C-to-U editing activity results in multiple mutagenic outcomes including …
single-stranded DNA C-to-U editing activity results in multiple mutagenic outcomes including …
The current toolbox for APOBEC drug discovery
MJ Grillo, KFM Jones, MA Carpenter, RS Harris… - Trends in …, 2022 - cell.com
Mutational processes driving genome evolution and heterogeneity contribute to immune
evasion and therapy resistance in viral infections and cancer. APOBEC3 (A3) enzymes …
evasion and therapy resistance in viral infections and cancer. APOBEC3 (A3) enzymes …
DNA deamination is required for human APOBEC3A-driven hepatocellular carcinoma in vivo
JA Naumann, PP Argyris, MA Carpenter… - International journal of …, 2023 - mdpi.com
Although the APOBEC3 family of single-stranded DNA cytosine deaminases is well-known
for its antiviral factors, these enzymes are rapidly gaining attention as prominent sources of …
for its antiviral factors, these enzymes are rapidly gaining attention as prominent sources of …