With the advent of recombinant DNA technology, cloning and expression of numerous
mammalian genes in different systems have been explored to produce many therapeutics …

INTRODUCTION Bacteria and archaea are frequently attacked by viruses (phages) and as a
result have developed multiple, sophisticated lines of active defense that can collectively be …

Publisher Summary The classical way of protein purification starting from a large number of
cells producing the protein using its authentic expression signals has been replaced by …

A series of plasmid-based expression vectors have been constructed allowing stable
intracellular expression of recombinant proteins in Bacillus subtilis strains. These expression …

Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein
production in Bacillus subtilis cells. Here, we present experimental data how to generate …

Abstract The Gram-positive bacterium Bacillus subtilis and some of its close relatives are
widely used for the industrial production of enzymes for the detergents, food, and beverage …

Two plasmid-based expression vectors have been constructed where one allows
intracellular production of recombinant proteins while the second directs the proteins into the …

ABSTRACT A novel technically compliant expression system was developed for
heterologous protein production in Bacillus subtilis with the aim of increasing product yields …

Squalene synthase (SQS) catalyzes the conversion of two farnesyl pyrophosphates to
squalene, an important intermediate in between isoprene and valuable triterpenoids. In this …

Bacterial small RNAs perform numerous regulatory roles, including acting as antitoxic
components in toxin–antitoxin systems. In type III toxin–antitoxin systems, small processed …