Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients
M Sattler, J Schleucher, C Griesinger - Progress in nuclear magnetic …, 1999 - Elsevier
6. 3D and 4D NOESY/ROESY experiments............................................ 146 6.1. 13C-and 15N-
edited NOESY/ROESY experiments.................................. 146 6.2. Filtered experiments for …
edited NOESY/ROESY experiments.................................. 146 6.2. Filtered experiments for …
THE USE OF 2H, 13C, 15N MULTIDIMENSIONAL NMR GTO STUDY THE STRUCTURE AND DYNAMICS OF PROTEINS
KH Gardner, LE Kay - Annual review of biophysics and …, 1998 - annualreviews.org
▪ Abstract During the past thirty years, deuterium labeling has been used to improve the
resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most …
resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most …
Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited …
JL Battiste, G Wagner - Biochemistry, 2000 - ACS Publications
To test whether distances derived from paramagnetic broadening of 15N heteronuclear
single quantum coherence (HSQC) resonances could be used to determine the global fold …
single quantum coherence (HSQC) resonances could be used to determine the global fold …
A robust and cost-effective method for the production of Val, Leu, Ile (δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins
NK Goto, KH Gardner, GA Mueller, RC Willis… - Journal of biomolecular …, 1999 - Springer
A selective protonation strategy is described that uses [3-2H] 13C α-ketoisovalerate to
introduce (1H-δ methyl)-leucine and (1H-γ methyl)-valine into 15N-, 13C-, 2H-labeled …
introduce (1H-δ methyl)-leucine and (1H-γ methyl)-valine into 15N-, 13C-, 2H-labeled …
The use of NMR methods for conformational studies of nucleic acids
SS Wijmenga, BNM Van Buuren - Progress in nuclear magnetic resonance …, 1998 - Elsevier
Nucleic acid molecules play a central role in cell biological processes. DNA's main role is to
act as the carrier of genetic information. Furthermore, DNA is transcribed into RNA by a …
act as the carrier of genetic information. Furthermore, DNA is transcribed into RNA by a …
NMR approaches for structural analysis of multidomain proteins and complexes in solution
NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …
multidomain proteins and complexes in solution. It plays a unique role in integrated …
Production and Incorporation of 15N, 13C, 2H (1H-δ1 Methyl) Isoleucine into Proteins for Multidimensional NMR Studies
KH Gardner, LE Kay - Journal of the American Chemical Society, 1997 - ACS Publications
The production of macromolecules labeled with NMR-active isotopes has greatly increased
the range of systems that are amenable to multidimensional solution NMR studies. Uniform …
the range of systems that are amenable to multidimensional solution NMR studies. Uniform …
Global folds of highly deuterated, methyl-protonated proteins by multidimensional NMR
The development of 15N, 13C, 2H multidimensional NMR spectroscopy has facilitated the
assignment of backbone and side chain resonances of proteins and protein complexes with …
assignment of backbone and side chain resonances of proteins and protein complexes with …
Methyl groups as probes of structure and dynamics in NMR studies of high‐molecular‐weight proteins
V Tugarinov, LE Kay - ChemBioChem, 2005 - Wiley Online Library
Solution NMR studies of biomolecules are typically limited by the molecules' molecular
weight. Here we report strategies based on the use of highly deuterated, methyl‐protonated …
weight. Here we report strategies based on the use of highly deuterated, methyl‐protonated …
Solution NMR spectroscopy beyond 25 kDa
LE Kay, KH Gardner - Current opinion in structural biology, 1997 - Elsevier
Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR
experiments and new deuterium-labeling strategies have significantly increased the scope …
experiments and new deuterium-labeling strategies have significantly increased the scope …