6. 3D and 4D NOESY/ROESY experiments............................................ 146 6.1. 13C-and 15N-
edited NOESY/ROESY experiments.................................. 146 6.2. Filtered experiments for …

▪ Abstract During the past thirty years, deuterium labeling has been used to improve the
resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most …

To test whether distances derived from paramagnetic broadening of 15N heteronuclear
single quantum coherence (HSQC) resonances could be used to determine the global fold …

A selective protonation strategy is described that uses [3-2H] 13C α-ketoisovalerate to
introduce (1H-δ methyl)-leucine and (1H-γ methyl)-valine into 15N-, 13C-, 2H-labeled …

Nucleic acid molecules play a central role in cell biological processes. DNA's main role is to
act as the carrier of genetic information. Furthermore, DNA is transcribed into RNA by a …

NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …

The production of macromolecules labeled with NMR-active isotopes has greatly increased
the range of systems that are amenable to multidimensional solution NMR studies. Uniform …

The development of 15N, 13C, 2H multidimensional NMR spectroscopy has facilitated the
assignment of backbone and side chain resonances of proteins and protein complexes with …

Solution NMR studies of biomolecules are typically limited by the molecules' molecular
weight. Here we report strategies based on the use of highly deuterated, methyl‐protonated …

Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR
experiments and new deuterium-labeling strategies have significantly increased the scope …