Pulsed-field gel electrophoresis (PFGE): A review of the “gold standard” for bacteria typing and current alternatives

H Neoh, XE Tan, HF Sapri, TL Tan - Infection, Genetics and Evolution, 2019 - Elsevier
Pulsed-field gel electrophoresis (PFGE) is considered the “gold standard” for bacteria typing.
The method involves enzyme restriction of bacteria DNA, separation of the restricted DNA …

[HTML][HTML] Meningococcal disease in North America: updates from the global meningococcal initiative

EJ Asturias, X Bai, JA Bettinger, R Borrow, DN Castillo… - Journal of Infection, 2022 - Elsevier
This review summarizes the recent Global Meningococcal Initiative (GMI) regional meeting,
which explored meningococcal disease in North America. Invasive meningococcal disease …

An implementation science approach to evaluating pathogen whole genome sequencing in public health

AS Ferdinand, M Kelaher, CR Lane, AG da Silva… - Genome medicine, 2021 - Springer
Background Pathogen whole genome sequencing (WGS) is being incorporated into public
health surveillance and disease control systems worldwide and has the potential to make …

Genomics for emerging pathogen identification and monitoring: Prospects and obstacles

V Vashisht, A Vashisht, AK Mondal, J Farmaha… - …, 2023 - mdpi.com
Emerging infectious diseases (EIDs) pose an increasingly significant global burden, driven
by urbanization, population explosion, global travel, changes in human behavior, and …

Establishment and evaluation of a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of Pseudomonas aeruginosa

H Tönnies, K Prior, D Harmsen… - Journal of clinical …, 2021 - Am Soc Microbiol
The environmental bacterium Pseudomonas aeruginosa, particularly multidrug-resistant
clones, is often associated with nosocomial infections and outbreaks. Today, core genome …

Full molecular typing of Neisseria meningitidis directly from clinical specimens for outbreak investigation

M Itsko, AC Retchless, SJ Joseph… - Journal of Clinical …, 2020 - Am Soc Microbiol
Neisseria meningitidis is a leading cause of bacterial meningitis and sepsis worldwide and
an occasional cause of meningococcal urethritis. When isolates are unavailable for …

Whole Genome Analyses Accurately Identify Neisseria spp. and Limit Taxonomic Ambiguity

M Khoder, M Osman, II Kassem, R Rafei… - International Journal of …, 2022 - mdpi.com
Genome sequencing facilitates the study of bacterial taxonomy and allows the re-evaluation
of the taxonomic relationships between species. Here, we aimed to analyze the draft …

Toward a global genomic epidemiology of meningococcal disease

AC Retchless, LAM Fox, MCJ Maiden… - The Journal of …, 2019 - academic.oup.com
Whole-genome sequencing (WGS) is invaluable for studying the epidemiology of
meningococcal disease. Here we provide a perspective on the use of WGS for …

Rapid and accurate SNP genotyping of clonal bacterial pathogens with BioHansel

G Labbé, P Kruczkiewicz, J Robertson… - Microbial …, 2021 - microbiologyresearch.org
Hierarchical genotyping approaches can provide insights into the source, geography and
temporal distribution of bacterial pathogens. Multiple hierarchical SNP genotyping schemes …

Enhancing meningococcal genomic surveillance in the meningitis belt using high-resolution culture-free whole-genome sequencing

M Itsko, N Topaz, S Ousmane-Traoré… - The Journal of …, 2022 - academic.oup.com
Rollout of meningococcal serogroup A conjugate vaccine in Africa started in 2010, aiming to
eliminate meningitis outbreaks, in meningitis belt countries. Since then, studies have been …