The interior lumen of acidic organelles (eg, endosomes, secretory granules, lysosomes and
plant vacuoles) is an important platform for modification, transport and degradation of …

Oligomerization of membrane proteins has received intense research interest because of
their importance in cellular signaling and the large pharmacological and clinical potential …

Genetically encoded fluorescent biosensors are powerful tools for monitoring biochemical
activities in live cells, but their multiplexing capacity is limited by the available spectral …

The approximately linear scaling of fluorescence quantum yield (ϕ) with fluorescence
lifetime (τ) in fluorescent proteins (FPs) has inspired engineering of brighter fluorophores …

Chimeric antigen receptor (CAR) T cell dysfunction is a major barrier to achieving lasting
remission in hematologic cancers, especially in chronic lymphocytic leukemia (CLL). We …

Background Genetically encoded calcium ion (Ca 2+) indicators (GECIs) are indispensable
tools for measuring Ca 2+ dynamics and neuronal activities in vitro and in vivo. Red …

Physiological changes in GTP levels in live cells have never been considered a regulatory
step of RAC1 activation because intracellular GTP concentration (determined by …

Fluorescence fluctuation spectroscopy has become a popular toolbox for non-disruptive
analysis of molecular interactions in living cells. The quantification of protein oligomerization …

Natural proteins exhibit rich structural diversity based on the folds of an invariably linear
chain. Macromolecular catenanes that cooperatively fold into a single domain do not belong …

Chemotactic bacteria not only navigate chemical gradients, but also shape their
environments by consuming and secreting attractants. Investigating how these processes …