Dimethyl sulfoxide-free cryopreservation for cell therapy: A review

L Weng, PR Beauchesne - Cryobiology, 2020 - Elsevier
Cell-based therapeutics promise to transform the treatment of a wide range of diseases
including cancer, genetic and degenerative disorders, or severe injuries. Many of the …

Cell theory, intrinsically disordered proteins, and the physics of the origin of life

VV Matveev - Progress in biophysics and molecular biology, 2019 - Elsevier
Cell theory, as formulated by Theodor Schwann in 1839, introduced the idea that the cell is
the main structural unit of living nature. Later, in solving the problem of cell multiplication …

A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation

K Tsuboyama, T Osaki, E Matsuura-Suzuki… - PLoS …, 2020 - journals.plos.org
Proteins are typically denatured and aggregated by heating at near-boiling temperature.
Exceptions to this principle include highly disordered and heat-resistant proteins found in …

Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins

AS Hsiao - International Journal of Molecular Sciences, 2024 - mdpi.com
Global climate change has caused severe abiotic and biotic stresses, affecting plant growth
and food security. The mechanical understanding of plant stress responses is critical for …

Evaluation of a bacterial group 1 LEA protein as an enzyme protectant from stress-induced inactivation

E Raga-Carbajal, G Espin, M Ayala… - Applied Microbiology …, 2022 - Springer
Late embryogenesis abundant (LEA) proteins are hydrophilic proteins that lack a well-
ordered tertiary structure and accumulate to high levels in response to water deficit, in …

Presence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of Aβ (1–42) in amino acid sequence independent manner

K Ikeda, S Suzuki, Y Shigemitsu, T Tenno, N Goda… - Scientific reports, 2020 - nature.com
The molecular shield effect was studied for intrinsically disordered proteins (IDPs) that do
not adopt compact and stable protein folds. IDPs are found among many stress-responsive …

The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region

T Kozono, H Sato, W Okumura, C Jogano… - Scientific reports, 2021 - nature.com
Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic
reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 …

Functional analysis of the N‐terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis

K Sasamoto, T Himiyama, K Moriyoshi… - FEBS Open …, 2022 - Wiley Online Library
Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866)
has an N‐terminal region (NTR; residues 23–135) between the signal sequence (residues 1 …

Using 1HN amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method

H Okazaki, N Matsuo, T Tenno, N Goda… - Protein …, 2018 - Wiley Online Library
This report describes a cost‐effective experimental method for determining an intrinsically
disordered protein (IDP) region in a given protein sample. In this area, the most popular (and …

A low-cost and easy-to-use cell preservation reagent for 4 C or room temperature sample storage

S Shankar, S Roy, A Geary-Teeter, GM Martin… - bioRxiv, 2019 - biorxiv.org
Current biospecimen storage and preservation methods no longer meet the demands of
basic research and clinical diagnostics. Biospecimen preservation methodology has not …