Shoot apical meristem: a sustainable explant for genetic transformation of cereal crops

MB Sticklen, HF Oraby - In Vitro Cellular & Developmental Biology-Plant, 2005 - Springer
Immature zygotic embryo has been the widely used explant source to develop embryogenic
callus lines, cell suspensions and protoplasts for transformation of cereal crops including …

Plant thin cell layers: a 40-year celebration

JA Teixeira da Silva, J Dobránszki - Journal of plant growth regulation, 2013 - Springer
The concept of a thin cell layer (TCL) was initially presented by Tran Thanh Van in two key
papers exactly 40 years ago. At that time, Nicotiana tabacum was the model plant used to …

Somatic embryogenesis in peach palm using the thin cell layer technique: induction, morpho-histological aspects and AFLP analysis of somaclonal variation

DA Steinmacher, NG Krohn, ACM Dantas… - Annals of …, 2007 - academic.oup.com
Abstract Background and Aims The thin cell layer (TCL) technique is based on the use of
very small explants and has allowed enhanced in vitro morphogenesis in several plant …

Somatic embryogenesis from immature peach palm inflorescence explants: towards development of an efficient protocol

DA Steinmacher, CR Clement, MP Guerra - Plant Cell, Tissue and Organ …, 2007 - Springer
Various factors affect the induction of somatic embryogenesis in peach palm (Bactris
gasipaes Kunth). Among these, both the type and level of auxins had the greatest influence …

Direct shoot regeneration and genetic fidelity analysis in finger millet using ISSR markers

G Atul Babu, A Vinoth, R Ravindhran - Plant Cell, Tissue and Organ …, 2018 - Springer
Finger millet (Eleusine coracana (L.) Gaertn.), an economically important food crop is
cultivated widely in the arid and semi-arid tropics of Africa and Asia. In the present study, an …

Direct plant regeneration from in vitro-derived shoot apical meristems of finger millet (Eleusine coracana (L.) Gaertn.)

L Satish, SA Ceasar, J Shilpha, AS Rency… - In Vitro Cellular & …, 2015 - Springer
A rapid and reproducible protocol for direct plant regeneration has been established using
in vitro-derived, actively growing shoot apical meristems (SAMs) of the genotype'CO (Ra) …

High-efficiency direct somatic embryogenesis and plant regeneration from leaf base explants of turmeric (Curcuma longa L.)

C Soundar Raju, A Aslam, A Shajahan - Plant Cell, Tissue and Organ …, 2015 - Springer
This study describes for the first time a simple and quick direct somatic embryogenesis and
plant regeneration protocol for turmeric (Curcuma longa L.) using leaf base explants …

Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis and ex vitro acclimatization …

B Bose, S Kumaria, H Choudhury, P Tandon - Physiology and Molecular …, 2017 - Springer
Abstract Malaxis wallichii (Lindl.) Deb, a small, perennial, monopodial, terrestrial orchid, is
endemic to tropical Himalayas at an altitude of 1200–2000 m asl. The pseudobulbs are …

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

A Ben Ghnaya, G Charles, M Branchard - Plant Cell, Tissue and organ …, 2008 - Springer
The present work describes a procedure that allows for the easy and rapid induction of
caulogenesis in four cultivars of Brassica napus L. from transversal Thin Cell Layers (tTCLs) …

Thin cell layer culture system in Lilium: Regeneration and transformation perspectives

DT Nhut, BV Le, JA Teixeira Da Silva… - In Vitro Cellular & …, 2001 - Springer
Lilies are one of the most valuable bulbous ornamental cut flower crops and are
commercially propagated by conventional scaling, which results in varied bulb quality. This …