Formation of chromatin subcompartments by phase separation

F Erdel, K Rippe - Biophysical journal, 2018 - cell.com
Chromatin is partitioned on multiple length scales into subcompartments that differ from
each other with respect to their molecular composition and biological function. It is a key …

Redox signaling by reactive electrophiles and oxidants

S Parvez, MJC Long, JR Poganik, Y Aye - Chemical reviews, 2018 - ACS Publications
The concept of cell signaling in the context of nonenzyme-assisted protein modifications by
reactive electrophilic and oxidative species, broadly known as redox signaling, is a uniquely …

Mouse heterochromatin adopts digital compaction states without showing hallmarks of HP1-driven liquid-liquid phase separation

F Erdel, A Rademacher, R Vlijm, J Tünnermann… - Molecular cell, 2020 - cell.com
The formation of silenced and condensed heterochromatin foci involves enrichment of
heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid …

A protein-tagging system for signal amplification in gene expression and fluorescence imaging

ME Tanenbaum, LA Gilbert, LS Qi, JS Weissman… - Cell, 2014 - cell.com
Signals in many biological processes can be amplified by recruiting multiple copies of
regulatory proteins to a site of action. Harnessing this principle, we have developed a …

Liquid–liquid phase separation in chromatin

K Rippe - Cold Spring Harbor perspectives in biology, 2022 - cshperspectives.cshlp.org
In eukaryotic cells, protein and RNA factors involved in genome activities like transcription,
RNA processing, DNA replication, and repair accumulate in self-organizing membraneless …

A comprehensive review of fluorescence correlation spectroscopy

L Yu, Y Lei, Y Ma, M Liu, J Zheng, D Dan, P Gao - Frontiers in physics, 2021 - frontiersin.org
Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of
molecular dynamics, and it has been widely applied in diverse fields, eg, biomedicine …

Evolutionary pathways and trajectories in antibiotic resistance

F Baquero, JL Martinez, V F. Lanza… - Clinical Microbiology …, 2021 - Am Soc Microbiol
Evolution is the hallmark of life. Descriptions of the evolution of microorganisms have
provided a wealth of information, but knowledge regarding “what happened” has precluded …

Open sesame: how transition fibers and the transition zone control ciliary composition

FR Garcia-Gonzalo, JF Reiter - Cold Spring Harbor …, 2017 - cshperspectives.cshlp.org
Cilia are plasma membrane protrusions that act as cellular propellers or antennae. To
perform these functions, cilia must maintain a composition distinct from those of the …

Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport

C Ma, S Wu, Y Zhou, HF Wei, J Zhang… - Angewandte …, 2021 - Wiley Online Library
A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL
imaging of intracellular structure and dynamic transport. This microscopy uses Ru (bpy) 32+ …

Polymer physics indicates chromatin folding variability across single-cells results from state degeneracy in phase separation

M Conte, L Fiorillo, S Bianco, AM Chiariello… - Nature …, 2020 - nature.com
The spatial organization of chromosomes has key functional roles, yet how chromosomes
fold remains poorly understood at the single-molecule level. Here, we employ models of …