Cell lysis is an essential step in many studies related to biology and medicine. Based on the
scale and medium that cell lysis is carried out, there are three main types of the cell lysis:(1) …

In recent years quantitative PCR (qPCR) detection methods have been widely utilised to
detect phytopathogenic fungi and oomycetes and have greatly contributed to the …

The genus Pythium is one of the most important groups of soilborne plant pathogens,
present in almost every agricultural soil and attacking the roots of thousands of hosts …

Verticillium dahliae is responsible for Verticillium wilt on a wide range of hosts, including
strawberry, on which low soil inoculum densities can cause significant crop loss …

Many species of fungi can cause disease in plants, animals and humans. Accurate and
robust detection and quantification of fungi is essential for diagnosis, modeling and …

Real‐time PCR protocols were developed to detect and discriminate 11 anastomosis groups
(AGs) of Rhizoctonia solani using ribosomal internal transcribed spacer (ITS) regions (AG‐1 …

Proper isolation and identification of Phytophthora species is critical due to their broad
distribution and huge impact on natural ecosystems throughout the world. In this study, five …

Detection and quantification of soil organisms using traditional methods (culture, plating and
microscopy) are difficult and do not always allow identification. In the last decade, real-time …

Fusarium oxysporum f. sp. spinaciae, causal agent of spinach Fusarium wilt, is an important
soilborne pathogen in many areas of the world where spinach is grown. The pathogen is …

The necrotrophic soilborne fungal pathogens Rhizoctonia solani AG8 and R. oryzae are
principal causal agents of Rhizoctonia root rot and bare patch of wheat in dryland cropping …