Plant promoters: their identification, characterization, and role in gene regulation

L Villao-Uzho, T Chávez-Navarrete, R Pacheco-Coello… - Genes, 2023 - mdpi.com
One of the strategies to overcome diseases or abiotic stress in crops is the use of improved
varieties. Genetic improvement could be accomplished through different methods, including …

Plant promoters and terminators for high-precision bioengineering

EG Brooks, E Elorriaga, Y Liu, JR Duduit… - BioDesign …, 2023 - spj.science.org
High-precision bioengineering and synthetic biology require fine-tuning gene expression at
both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by …

Strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding

J Zhou, X Luan, Y Liu, L Wang, J Wang, S Yang, S Liu… - Plants, 2023 - mdpi.com
Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has
become increasingly mature and is being widely used for crop improvement. The application …

Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters

SZ Shah, TN Perry, A Graziadei, V Cecatiello… - Nature …, 2025 - nature.com
Abstract RNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6
small nuclear RNA (snRNA). At U6 snRNA genes, Pol III is recruited by the snRNA Activating …

[HTML][HTML] Investigating cold tolerance mechanisms in rice seedlings: Alternative splicing, promoter analysis, and their applications for marker development

P Summat, K Tongmark, S Chakhonkaen, N Sangarwut… - Plant Stress, 2024 - Elsevier
Cold stress harms rice seedlings, causing yield reduction. Enhancing cold tolerance at the
seedling stage is crucial for rice breeding. OsFH10, OsFER1, ONAC045, and OsProT were …

Efficient gene editing of a model fern species through gametophyte-based transformation

W Jiang, F Deng, M Babla, C Chen, D Yang… - Plant …, 2024 - academic.oup.com
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated nuclease (Cas) system allows precise and easy editing of genes in many plant …

Recalcitrance to transformation, a hindrance for genome editing of legumes

VM Nivya, JM Shah - Frontiers in Genome Editing, 2023 - frontiersin.org
Plant genome editing, a recently discovered method for targeted mutagenesis, has emerged
as a promising tool for crop improvement and gene function research. Many genome-edited …

Recent developments in CRISPR/Cas9 genome-editing technology related to plant disease resistance and abiotic stress tolerance

İ Erdoğan, B Cevher-Keskin, Ö Bilir, Y Hong, M Tör - Biology, 2023 - mdpi.com
Simple Summary Pests and diseases, along with environmental factors, significantly
contribute to yield losses in crop production. Considering the detrimental impact of …

Application of an endogenous pGhαGloA promoter in CRISPR/Cas12a system for efficient genome editing to creat glandless cotton germplasm

C Li, Z Tuerxun, Y Yang, X Li, F Hui, J Li, Z Liu… - Journal of Integrative …, 2024 - Elsevier
The efficient genome editing tool (the CRISPR/Cas12a system) has been used in research
on plant functionional genomics and improvement of agronomic traits. In this study …

Gene editing tool kit in millets: present status and future directions

V Sapara, M Khisti, K Yogendra, PS Reddy - The Nucleus, 2024 - Springer
Abstracts Millets, the sixth most-grown group of crops in the drylands, support the livelihood
of many small-holder farmers in the region. Being one of the most nutritious groups of crops …