Click chemistry is an immensely powerful technique for the fast and efficient covalent
conjugation of molecular entities. Its broad scope has positively impacted on multiple …

The Huisgen 1, 3-dipolar cycloaddition reaction of organic azides and alkynes1, 2 has
gained considerable attention in recent years due to the introduction in 2001 of Cu (1) …

The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The
copper catalysed alkyne–azide cycloaddition (CuAAC) reaction is the method of choice for …

The CuI‐catalyzed azide–alkyne cycloaddition (CuAAC) has been established as a powerful
coupling technology for the conjugation of proteins, nucleic acids, and polysaccharides …

Biochemical strategies that use a combination of synthetic oligonucleotides, thermostable
DNA polymerases, and DNA ligases can produce large DNA constructs up to 1 megabase in …

The CuAAC “click” reaction has developed as one of the most useful and widely employed
reactions in ligation within polymer chemistry. This is due to the unique properties of the Cu …

A triazole mimic of a DNA phosphodiester linkage has been produced by templated
chemical ligation of oligonucleotides functionalized with 5′-azide and 3′-alkyne. The …

The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase
synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length …

Templated DNA strand ligation by the ring-strain promoted alkyne–azide [3+ 2] cycloaddition
reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It …

DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click
DNA ligation between oligonucleotides with 3′-AZT and 5′-propargylamido dT and …