During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

Transcription elongation requires elaborate coordination between the transcriptional
machinery and chromatin regulatory factors to successfully produce RNA while preserving …

During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a
chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS …

SPT6 is a histone chaperone that tightly binds RNA polymerase II (RNAPII) during
transcription elongation. However, its primary role in transcription is uncertain. We used …

Histone modifications coupled to transcription elongation play important roles in regulating
the accuracy and efficiency of gene expression. The monoubiquitylation of a conserved …

The kinetochore is a mega-dalton protein assembly that forms within centromeric regions of
chromosomes and directs their segregation during cell division. Here we review cell cycle …

The human genome encodes large numbers of non-coding RNAs, including divergent
antisense transcripts at transcription start sites (TSSs). However, molecular mechanisms by …

ABSTRACT The casein kinase 2 (CK2) complex has garnered extensive attention over the
past decades as a potential therapeutic target for diverse human diseases, including cancer …

Temperature-sensitive (TS) missense mutants have been foundational for characterization
of essential gene function. However, an unbiased approach for analysis of biochemical and …

Spn1/Iws1 is a conserved protein involved in transcription and chromatin dynamics, yet its
general in vivo requirement for these functions is unknown. Using a Spn1 depletion system …