Abstract The 4D Nucleome Network aims to develop and apply approaches to map the
structure and dynamics of the human and mouse genomes in space and time with the goal …

A long-standing mystery in the field of Polycomb and Trithorax regulation is how these
proteins, which are highly conserved between flies and mammals, can regulate several …

DNA double-strand break (DSB) repair is mediated by multiple pathways. It is thought that
the local chromatin context affects the pathway choice, but the underlying principles are …

It is largely unclear whether genes that are naturally embedded in lamina-associated
domains (LADs) are inactive due to their chromatin environment or whether LADs are …

CRISPR activation (CRISPRa) is an important tool to perturb transcription, but its
effectiveness varies between target genes. We employ human pluripotent stem cells with …

Enhancers control the establishment of spatiotemporal gene expression patterns throughout
development. Over the past decade, the development of new technologies has improved our …

Selective recruitment and concentration of signalling proteins within membraneless
compartments is a ubiquitous mechanism for subcellular organization,–. The dynamic flow of …

Transcriptionally inactive genes are often positioned at the nuclear lamina (NL), as part of
large lamina‐associated domains (LADs). Activation of such genes is often accompanied by …

Detailed genomic contact maps have revealed that chromosomes are structurally organized
in megabase-sized topologically associated domains (TADs) that encompass smaller …

Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9
activity is crucial to design effective gene editing assays. However, it is challenging to profile …