How do intrinsically disordered protein regions encode a driving force for liquid–liquid phase separation?
Highlights•Not all IDRs undergo liquid–liquid phase separation.•IDRs are not necessary for
protein phase separation.•A stickers-and-spacers framework helps conceptualizing IDR …
protein phase separation.•A stickers-and-spacers framework helps conceptualizing IDR …
Theories for sequence-dependent phase behaviors of biomolecular condensates
Liquid–liquid phase separation and related condensation processes of intrinsically
disordered proteins (IDPs), proteins with intrinsically disordered regions, and nucleic acids …
disordered proteins (IDPs), proteins with intrinsically disordered regions, and nucleic acids …
LASSI: A lattice model for simulating phase transitions of multivalent proteins
Many biomolecular condensates form via spontaneous phase transitions that are driven by
multivalent proteins. These molecules are biological instantiations of associative polymers …
multivalent proteins. These molecules are biological instantiations of associative polymers …
Simulation of FUS protein condensates with an adapted coarse-grained model
Disordered proteins and nucleic acids can condense into droplets that resemble the
membraneless organelles observed in living cells. MD simulations offer a unique tool to …
membraneless organelles observed in living cells. MD simulations offer a unique tool to …
Complete phase diagram for liquid–liquid phase separation of intrinsically disordered proteins
A number of intrinsically disordered proteins have been shown to self-assemble via liquid–
liquid phase separation into protein-rich and dilute phases. The resulting coacervates can …
liquid phase separation into protein-rich and dilute phases. The resulting coacervates can …
Comparative roles of charge, π, and hydrophobic interactions in sequence-dependent phase separation of intrinsically disordered proteins
Endeavoring toward a transferable, predictive coarse-grained explicit-chain model for
biomolecular condensates underlain by liquid–liquid phase separation (LLPS) of proteins …
biomolecular condensates underlain by liquid–liquid phase separation (LLPS) of proteins …
Clustering of aromatic residues in prion-like domains can tune the formation, state, and organization of biomolecular condensates: published as part of the …
In immature oocytes, Balbiani bodies are conserved membraneless condensates implicated
in oocyte polarization, the organization of mitochondria, and long-term organelle and RNA …
in oocyte polarization, the organization of mitochondria, and long-term organelle and RNA …
Cell cycle-specific phase separation regulated by protein charge blockiness
H Yamazaki, M Takagi, H Kosako, T Hirano… - Nature Cell …, 2022 - nature.com
Dynamic morphological changes of intracellular organelles are often regulated by protein
phosphorylation or dephosphorylation,,,,–. Phosphorylation modulates stereospecific …
phosphorylation or dephosphorylation,,,,–. Phosphorylation modulates stereospecific …
Conformational ensembles of intrinsically disordered proteins and flexible multidomain proteins
FE Thomasen, K Lindorff-Larsen - Biochemical Society …, 2022 - portlandpress.com
Intrinsically disordered proteins (IDPs) and multidomain proteins with flexible linkers show a
high level of structural heterogeneity and are best described by ensembles consisting of …
high level of structural heterogeneity and are best described by ensembles consisting of …
Narrow equilibrium window for complex coacervation of tau and RNA under cellular conditions
The mechanism that leads to liquid-liquid phase separation (LLPS) of the tau protein, whose
pathological aggregation is implicated in neurodegenerative disorders, is not well …
pathological aggregation is implicated in neurodegenerative disorders, is not well …