Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology

SA Sripada, E Barbieri, S Shastry… - Analytical …, 2024 - ACS Publications
The limited biomolecular and functional stability of lentiviral vectors (LVVs) for cell therapy
poses the need for analytical tools that can monitor their titers and activity throughout the …

The downstream bioprocess toolbox for therapeutic viral vectors

R Kilgore, A Minzoni, S Shastry, W Smith… - … of Chromatography A, 2023 - Elsevier
Viral vectors are poised to acquire a prominent position in modern medicine and
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …

Peptide ligands for the universal purification of exosomes by affinity chromatography

RE Kilgore, BD Moore, SA Sripada… - Biotechnology and …, 2024 - Wiley Online Library
Exosomes are gaining prominence as vectors for drug delivery, vaccination, and
regenerative medicine. Owing to their surface biochemistry, which reflects the parent cell …

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

S Shastry, E Barbieri, A Minzoni, W Chu… - … of Chromatography A, 2024 - Elsevier
Adeno-associated viruses (AAVs) have emerged as a prominent family of vectors for gene
delivery, providing therapeutic options to diseases once deemed incurable. At the same …

Affinity chromatography for virus-like particle manufacturing: challenges, solutions, and perspectives

J Ma, Z Tian, Q Shi, X Dong, Y Sun - Journal of Chromatography A, 2024 - Elsevier
The increasing medical application of virus-like particles (VLPs), notably vaccines and viral
vectors, has increased the demand for commercial VLP production. However, VLP …

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

Y Wu, E Barbieri, RE Kilgore, BD Moore, W Chu… - … of Chromatography A, 2024 - Elsevier
Adenovirus (AdVs) is the viral vector of choice in vaccines and oncolytic applications owing
to its high transduction activity and inherent immunogenicity. For decades, AdV isolation has …

Discovery of broad-spectrum high-affinity peptide ligands of spike protein for the vaccine purification of SARS-CoV-2 and Omicron variants

J Ma, Y Huang, G Jia, X Dong, Q Shi, Y Sun - International Journal of …, 2024 - Elsevier
To combat with emerging SARS-CoV-2 variants of concern (VOCs), we report the
identification of a set of unique HWK-motif peptide ligands for the receptor-binding domain …