Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure
of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning …

The visualization of cellular ultrastructure over a wide range of volumes is becoming
possible by increasingly powerful techniques grouped under the rubric “volume electron …

Mechanisms of therapeutic resistance and vulnerability evolve in metastatic cancers as
tumor cells and extrinsic microenvironmental influences change during treatment. To …

Scanning electron microscopy (SEM) use in the biomedical sciences has traditionally been
used for characterisation of cell and tissue surface topography. This paper demonstrates the …

Despite recent progress in the characterization of tumour components, the tri-dimensional
(3D) organization of this pathological tissue and the parameters determining its internal …

Glioblastoma (GBM), the most common human brain tumor, has been notoriously resistant to
treatment. As a result, the dismal overall survival of GBM patients has not changed over the …

New developments in electron microscopy technology, improved efficiency of detectors, and
artificial intelligence applications for data analysis over the past decade have increased the …

Focused ion beam-scanning electron microscopy (FIB-SEM) images can provide a detailed
view of the cellular ultrastructure of tumor cells. A deeper understanding of their organization …

Structural observations are essential for the advancement of life science. Volume electron
microscopy has recently realized remarkable progress in the three-dimensional analyses of …